血管内皮生长因子及色素上皮衍生因子在乳腺癌中的表达及临床意义

Expressions of vascular endothelial growth factor and pigment epithelium-derived factor in breast cancer and their clinical significance

目的探讨血管内皮生长因子(VEGF)、色素上皮衍生因子(PEDF)在乳腺癌组织中的表达水平及其与乳腺癌病理特征的关系。方法采用Envision免疫组织化学方法对20例乳腺良性病变组织(纤维腺瘤)、85例乳腺浸润性导管癌组织中的VEGF、PEDF和CD34表达情况进行检测。CD34表达反映微血管密度(MVD)。计数资料采用χ2检验,Spearman等级相关分析方法进行相关性分析。结果 VEGF在乳腺癌组织中的阳性表达率为71.7%(61/85),高于在乳腺良性组织中的阳性表达率40%(8/20),差异有统计学意义(χ2=9.959,P=0.002);PEDF在乳腺癌组织中的阳性表达率为41.2%(35/85),低于在乳腺良性组织中的阳性表达率90%(18/20),差异有统计学意义(χ2=19.683,P=0.000)。在乳腺癌组织中VEGF与PEDF表达呈负相关(r=-0.365,P=0.019)。乳腺癌组织中VEGF表达与肿瘤直径(χ2=26.31,P=0.000)、TNM分期(χ2=5.428,P=0.020)、淋巴结转移有关(χ2=5.368,P=0.021);PEDF表达与TNM分期(χ2=8.584,P=0.003)、肿瘤直径(χ2=11.079,P=0.001)、绝经状态(χ2=4.507,P=0.034)、ER状态有关(χ2=3.974,P=0.046)。MVD值在PEDF阴性组高于阳性组(38.67±6.52比22.56±5.16,Z=-0.984,P=0.000),在VEGF阳性组则高于阴性组(38.78±6.28比25.36±5.12,Z=-0.972,P=0.000)。结论乳腺癌组织中存在PEDF、VEGF的表达相关性,且与MVD相关,对认识乳腺癌的生物学特性以及指导乳腺癌的诊疗具有重要意义。

Objective To investigate the expressions of vascular endothelial growth factor （ VEGF ） and pigment epithelium-derived factor （ PEDF ） in breast cancer tissue , and their relationship with the clinicopathological characteristics of breast cancer patients .Methods The expressions of VEGF , PEDF and CD34 were detected in 20 cases of benign breast diseases （ fibroadenoma ） and 85 cases of invasive ductal carcinoma by Envision immunohistochemical method .CD34 expression reflected microvessel density （ MVD）. The numeration data were analyzed by Chi-square test, and Spearman rank test was used for correlation analysis.Results The positive expression rate of VEGF was 71.7% （ 61/85 ） in breast cancer tissue , significantly higher than 40%（8/20）in breast benign tissue （χ2=9.959, P=0.002）;The positive expression rate of PEDF was 41.2%（ 35/85 ） in breast cancer tissue , significantly lower than 90%（ 18/20 ） in breast benign tissue （χ2=19.683 , P=0.000 ）; VEGF expression was negatively correlated with PEDF expression＆amp;nbsp;（ r=-0.365 , P=0.019 ） .In invasive ductal carcinoma , the expression of VEGF was significantly related with tumor diameter （χ2=26.31 ,P=0.000 ） , TNM stage （χ2=5.428 ,P=0.020 ） , and lymphatic metastasis （χ2=5.368 ,P=0.021 ）; the expression of PEDF was significantly related with tumor diameter （χ2=11.079 , P=0.001 ） , TNM stage （χ2=8.584 , P=0.003 ） , menstruation status （χ2=4.507 , P=0.034 ） , and ER status （χ2=3.974 , P=0.046 ） .The MVD value in PEDF negative was higher than that in PEDF positive group （38.67±6.52 vs 22.56±5.16,Z=-0.984,P=0.000）;The MVD value in VEGF positive was higher than that in VEGF negative group （ 38.78 ±6.28 vs 25.36 ±5.12 , Z=-0.972 , P=0.000 ） .Conclusion There is a correlation between PEDF and VEGF expression in breast cancer tissue , and the expressions of the two factors are also related to MVD, which may provide reference to the physicians in recognizing the biological characteristics of breast cancer and guiding the diagnosis and treatment of breast cancer .