水稻转录因子Os1137的克隆及其耐盐性分析

Cloning of Rice Transcription Factor Os1137 and Analysis on Its Salts Tolerance

通过PCR方法从水稻cDNA中克隆得到转录因子Os1137的编码基因,构建由ubi启动子驱动的该基因的植物表达载体p3300-Os1137,并用农杆菌介导法将其导入烟草,研究不同盐浓度下转基因烟草的生理指标,分析其抗盐性。结果表明,将该基因导入烟草植株中能够显著抑制NaCl胁迫下植物体内MDA含量的增加,并能够提高植物体内的脯氨酸含量。该基因能够提高转基因烟草植株的抗盐性。

In this study, the encoded gene of transcription factor Os 1137 was isolated from the cDNA of rice by PCR amplification. The gene was driven by Ubi promoter to construct plant expression vector p3300-Os1137, which was introduced into tobacco via agrobacterium-mediated transformation. The salt tolerance of the transgenic plant was analyzed by testing physiological changes of the transgenic tobacco plants under different salt stresses. Results showed that less increment of MDA contents and more of proline contents in the transgenic plants respectively in high salinity were obtained. Quantitative test showed that the salt tolerance of tobacco plants was improved by this gene expression.