石斛合剂对老年糖尿病大鼠GCK、GLUT-2的影响

Effects of Shihu Heji on expressions of GCK、GLUT-2 of rats with senile diabetes

目的:观察石斛合剂(DC)对老年糖尿病大鼠血糖、GSP水平以及GCK、GLUT-2表达的影响,探讨其治疗糖尿病的作用机制。方法:Wistar雌性大鼠分为正常组(9只)、模型组(12只)、石斛合剂低剂量组(12只)、石斛合剂高剂量组(12只)、二甲双胍对照组(10只),检测各组大鼠血糖、GSP水平,取胰腺组织,免疫组化检测GLUT-2、GCK阳性面积、IOD。结果:与模型组比较,石斛合剂低、高剂量组血糖、GSP水平显著降低(P<0.05,P<0.01),GCK、GLIT-2阳性面积、IOD显著升高(P<0.05,P<0.01)。结论:DC具有使胰岛GCK与GLUT-2表达上调的作用,可能是石斛合剂调节糖代谢作用的机制之一。

Objective：To observe the effects of Shihu Heft （DC）on contents of blood sugar, GSP and expressions of GCK, GLUT-2 of rats with senile diabetes,and approach its mechanism in treating diabetes. Methods：Wistar female rats were randomly divided into normal control group（n=9 ）, model group（n= 12 ）, metformin group（n= 10）, large dosage group（ n=12 ） and small dosage group（n=12）of DC. Then contents of blood sugar, GSP, positive areas and IOD of GCK,GLUT-2 of pan- creatic tissue were detected. Results ： Compared with model group,contents of blood sugar, GSP were decreased, positive areas and IOD of GCK,GLUT-2 of pancreatic tissue were increased in large dosage and small dosage of DC group（P〈0.05, P〈0.01 ）. Conclusion ： DC can regulate glucose metabolism through up-regulating the expressions of GCK and GLUT-2 in pancreatic island.