复配精油对采后葡萄灰霉菌抑制作用的研究

Antibacterial effect of compound essential oil on postharvest Botrytis cinerea

以葡萄灰霉菌为病原菌,采用菌丝生长速率法研究3种复配植物精油（肉桂醛/柠檬醛、肉桂醛/丁香酚和柠檬醛/丁香酚）的协同抑菌效果,通过测定细胞溶出物的释放量和细胞膜渗透性,对其协同抑菌作用机理进行初步分析。结果表明：不同复配植物精油处理对采后葡萄灰霉菌的抑制活性显著（p〈0.05）,使细胞溶出物释放量和细胞膜渗透性显著增加（p〈0.05）。以500μL/L肉桂醛/柠檬醛协同抑制效果最明显,在培养过程中肉桂醛/柠檬醛的EC50值为67.46~101.31μL/L,抑菌效果最稳定,抑菌率达93.75%;肉桂醛/柠檬醛协同抑菌作用可能在于破坏灰霉菌的细胞膜,使内溶物和离子外流而导致细胞的死亡。

The synergistic antibacterial effect of three compound essential oils （cinnamaldehyde/citral, cinnamaldehyde/eugenol, citral/eugenol）on Botrytis cinerea was studied through mycelial growth rate method.The synergistic antibacterial mechanism was preliminarily analyzed through measuring the cell constituent release and cell membrance potential. The results indicated that the antibacterial effect of the compound essential oils on postharvest Botrytis cinerea was higher significantly（p 〈 0.05）than the individual treatmenLAnd the cell constituent release and cell membrance potential was higher significantly（ p 〈 0.05）.The 500μL/L cinnamaldehyde/citral had the best effect.During the time of culture, ECso was 67.46-101.31μL/L for cinnamaldehyde/citraL.The antibacterial effect of cinnamaldehyde/citral was the most stable. The antibacterial effect rate was 93.75%.The synergistic antibacterial effect of cinnamaldehyde/citral could destruct the cell membrane of Botrytis cinere＆ Due to the outflow of cell constituent and ion the cell of Botrytis cinerea died.