产内切菊粉酶的海洋放线菌菌株的筛选与鉴定

Screening and identification of marine actinomycetes producing endoinulinase

获得高活性内切菊粉酶微生物菌株是以菊粉生产低聚果糖的基础。本研究采用以菊粉为唯一碳源的选择培养基从鸭绿江滨海湿地海泥样品中筛选获得14株产菊粉酶菌株。通过摇瓶复筛,获得3株产较高活力内切菊粉酶的放线菌菌株,其酶活力分别为14.13、18.24、28.52U/m L。通过16S rRNA测序分析和形态观察确定该3株菌分别为孔雀石褐链霉菌(Streptomyces malachitofuscus)、棘孢小单孢菌(Micromonospora echinospora)、锂链霉菌(Streptomyces carpaticu)。其产酶活性均高于目前已报道产内切菊粉酶放线菌菌株,为内切菊粉酶工业化生产提供了依据。

Fructooligosaccharides are produced by the inulin cut with endoinulinase and microorganism is the main sources for endoinulinase.ln present work, 14 strains microorganism producing inulinase were acquired from Yalu River seacoast wetland samples with selective medium containing inulin as a sole carbon source.Meanwhile,three strains with high endoinulinase activity were obtained, and their enzyme activities were 14.13, 18.24 and 28.52U/mL, respectively.Through morphological and analysis of the 16S rRNA revealed that the strain S502, S503 andS505 identified as Streptomyces malachitofuscus, Micromonospora echinospora and Streptomyces carpaticus, respectively.The endoinulinase activity by actinomycete is higher than that reported in the literatures.Furthermore, as a new potential candidate for industrial endoinulinase production.