摘要
为了研究噬菌体作为天然生物防控剂应用于食品中的前景,本实验从水产品市场的污水中采用双层平板法分离纯化出一株烈性副溶血性弧菌噬菌体SHOU24,测定其生理特性,并探讨其在即食虾中的抑菌效果。结果表明：该噬菌体只对含有毒力基因tdh的副溶血性弧菌有裂解能力。电镜观察其形态表明该噬菌体属于长尾噬菌体科。SHOU24的最适p H为7~8。在50℃温度以下,存活率很高。抑菌实验结果表明该噬菌体在常温条件下对即食虾中副溶血性弧菌的生长具有良好的抑制作用,能使副溶血性弧菌的菌浓度下降1log CFU/g。本研究为副溶血性弧菌噬菌体SHOU24作为抑菌剂应用于食品中提供了一定的理论基础。
Aimed at using bacteriophage as natural anti-bacteria,this study isolated a lytic Vibrio parahaemolyticus phage through sewage from aquatic market by double-layer method and it was designated as SHOU24,and pH and thermal stability were tested.Phage SHOU24 was specific lytic to Vibrio parahaemolyticus with tdh gene.The phage belonged to Siphoviridae by transmission electron microscope （TEM）.The most suitable pH for phage SHOU24 was 7-8 while it could live at pH between 3-11.The survival rate was high under 50℃ condition.In anti- bacteria on ready-to-eat shrimp experiment, the bacteria amount dropped by t logCFU/g at 25℃.This study provided theory foundation for using phage SHOU24 as bacterial biocontrol agent.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第21期175-178,共4页
Science and Technology of Food Industry
基金
上海市科学技术委员会部分地方院校能力建设项目(11310501100)
上海市科学技术委员会科技创新行动计划项目(12391901300)
交叉学科项目(B5201120040)
上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号)
关键词
噬菌体
副溶血性弧菌
抑菌
即食对虾
Bacteriophage
Vibrio parahaemolyticus
anti- bacteria
ready- to- eat shrimp
