右归丸对二甲基亚硝胺诱导大鼠肝纤维化损伤的防护作用

Protection effect of Yougui pill extract on nitrosodimethylamine-induced liver damage rat

目的:观察右归丸对二甲基亚硝胺(DMN)诱导的肝纤维化大鼠的防护作用。方法:Wistar雄性大鼠随机分为正常组,模型组,治疗组3组,除正常组外,其余两组大鼠以DMN复制肝损伤模型,于造模第2周开始,用右归丸给予治疗组大鼠灌胃治疗。动态记录各组大鼠体重,死亡情况,计算腹水率;肝组织做天狼星红胶原染色,羟脯氨酸(Hyp)含量测定及血清丙氨酸氨基转移酶(ALT)检测。结果:与DMN模型组比较,治疗组大鼠体重下降有所恢复,死亡率明显降低,腹水率减小,肝组织胶原面积减小(P<0.05);血清ALT显著降低(P<0.05)。结论:右归丸对于DMN诱导的大鼠肝纤维化有显著的防护作用。

Objective： To observe the effect of You gui pill extract on Nitrosodimethylamine （ DMN） -induced liver fibro-sis damage rat.Methods： Male Wistar rats were randomly divided into normal group, model group, treatment groups;DMN was used to induce liver damage on rats, and treatment groups were given the Yougui pill extract by gastric intervention.Observe the rats′weight, mortality, and cirrhosis with ascites.Sirius red staining of collagen, hydroxyproline （ Hyp） content determination were to observe the liver tissue pathological changes, and serum alanine transaminase （ ALT ） test was to examine liver function.Results：Compared with DMN model group, weight loss of Yougui pill treatment group rats recovered, mortality and cir-rhosis with ascites declined obviously, liver tissue collagen area of treatment group decreased （P〈0.05）, and serum ALT de-creased significantly （ P〈0.05） .Conclusion： Yougui pill can significantly protect rats from liver fibrosis damage induced by DMN.