超声法优化金叶女贞叶中总黄酮的提取

Optimum Extraction of Total Flavonoids from Ligustrum Lucidum cv.by Ultrasonic Wave

以金叶女贞叶为材料,寻找其中总黄酮的超声提取的最佳条件.以总黄酮含量为考察指标,在单因素实验的基础上,采用L9(34)正交实验,超声提取研究了乙醇浓度、超声提取时间、超声功率、超声温度对提取金叶女贞叶中总黄酮的影响.从实验结果分析,影响超声提取效果的因素为乙醇浓度>超声时间>提取功率>提取温度,结果表明粒度为40目,乙醇浓度为65%,超声时间为75 min,超声功率为400 W,提取温度为50℃时总黄酮得率最高.

An ultrasonic method for the extraction of total flavonoids from Ligustrum Vicaryi Hort. was investigated and optimized. Using single factor and L9（ 3^4） orthogonal design, the effects of ethanol concentrations, ultrasonic time, ultrasonic power and extraction temperature were considered. The impact will activate by ethanol concentrations, ultrasonic time, ultrasonic power and extraction temperature. The optimum extracting conditions of total flavonoids are ： mesh number 40, extraction time 75 min, extraction temperature 50 ℃, ultrasonic power 400 W and ethanol concentrations 65%. Under these conditions the yield of total flavonoids can reach the highest.