血小板膜糖蛋白Ibα基因多态性与复发性脑梗死的分析研究

Platelet Glycoprotein Ibα VNTR Gene Polymorphisms and Recurrent Cerebral Infarction

目的探讨血小板膜糖蛋白Ibα可变数目串联重复序列(VNTR)基因多态性,了解其等位基因和基因型与复发性脑梗死的相关性。方法选择2010年2月至2011年1月淄博市中心医院神经内科收治的110例复发性脑梗死患者作为复发组,105例初发脑梗死患者为初发组,采用聚合酶链反应扩增目的片段,根据产物的长度判断VNTR基因多态性。观察比较两组等位基因及基因型出现频率的差异。结果 B等位基因于复发组出现频率显著高于初发组(χ2=10.059,P=0.002),基因型以CC、CD多见,BC在复发组显著高于初发组(χ2=12.208,P=0.001)。Logistic回归分析结果显示,B等位基因、BC基因型、原发性高血压、糖尿病是脑梗死复发的独立危险因素(P<0.05)。结论血小板膜糖蛋白Ibα基因多态性与复发性脑梗死密切相关,B等位基因和BC基因型是其致病危险因素。

Objective To study the gene polymorphisms of platelet membrane glycoprotein Ⅰb α variable number tandem repeats（VNTR）,understand the correlation between its alleles and genotypes and the recurent cerebral infarction.Methods Choosing from Zibo Central Hospital during Feb.2010 and Jan.2011,110 cases of neurology patients with recurrent cerebral infarction were included as recurrence group,105 patients with incipient cerebral infarction were included as incipient group,using polymerase chain reaction to amplify the target fragments,VNTR gene polymorphism was judged according to the length of the product.The allele and genotype frequency difference of the two groups were observed and compared.Results B allele frequency in the recurrent group was significantly higher than that of the incipient group（χ2 =10.059,P =0.002）,with CC genotype,CD,BC in the recurrence group was obviously higher than the incipient group （χ2 =12.208,P=0.001）.Logistic regression analysis showed：B allele,genotype BC,hypertension were independent risk factors of the recurrence of cerebral infarction（P 〈 0.05）.Conclusion Platelet membrane glycoprotein Ⅰb α gene polymorphism and recurrent cerebral infarction is closely related,and B alleles and BC genotype are the risk factors.