3种仪器检测糖化血红蛋白结果分析

Comparison of glycosylated hemoglobin levels detected by 3 kinds of analytic instruments

目的比较3种仪器检测糖化血红蛋白的结果。方法分别采用D-10全自动糖化血红蛋白分析仪(离子交换色谱法)、HA-8160全自动糖化血红蛋白分析仪(亲和色谱法)和7170A全自动生化分析仪(免疫比浊法)检测75例全血样本,对检测结果进行方差齐性检验,方差齐后,进行单因素方差分析和相关性分析。结果 D-10全自动糖化血红蛋白分析仪和HA-8160全自动糖化血红蛋白分析仪测定结果呈正相关(r2=0.996),回归方程为Y=0.953 X+0.519;D-10全自动糖化血红蛋白分析仪和7170A全自动生化分析仪测定结果呈正相关(r2=0.996),回归方程为Y=0.925 X+0.576;HA-8160全自动糖化血红蛋白分析仪和7170A全自动生化分析仪测定结果呈正相关(r2=0.998),回归方程为Y=0.969 X+0.081。结论在保证实验室质量控制的前提下,均可以采用以上3种不同的仪器检测糖化血红蛋白。

Objective To compare levels of glycosylated hemoglobin detected by 3 kinds of analytic instruments .Methods 75 samples were measured by D-10 glycosylated hemoglobin automatic analyzer（ionexchange chromatography） ,HA-8160 glycosylated hemoglobin automatic analyzer（affinity chromatography） and 7170A automatic analyzer（immune turbidimetry） .Results were tested by the homogeneity of variance ,the one-way analysis of variance and correlation analysis .Results There was a positive correlation between D-10 glycosylated hemoglobin automatic analyzer and HA-8160 glycosylated hemoglobin automatic analyzer（r2 =0 .996） , the linear equation was Y=0 .953X＋0 .519 .There was a positive correlation between D-10 glycosylated hemoglobin automatic ana-lyzer and 7170A automatic analyzer（r2 =0 .996） ,the linear equation was Y=0 .925X＋0 .576 .There was a positive correlation be-tween HA-8160 glycosylated hemoglobin automatic analyzer and 7170A automatic analyzer（r2 =0 .998） ,the linear equation was Y=0 .969 X＋0 .081 .Conclusion In the premise of quality control in laboratory ,three different instrument can use to detect the level of glycosylated hemoglobin .