复方藤梨根制剂抑制小鼠结肠癌人工转移实验研究

Inhibitory Effect of Fufang Tengligen Mixture on Metastatic Lung Cancer from Colon Cancer in Mice

目的观察复方藤梨根制剂对小鼠结肠癌人工转移模型的病理生理、大体解剖、分子影像及病理的影响。方法 BALB/c小鼠50只,随机分成复方藤梨根高、中、低剂量组、模型组、空白对照组5组,每组10只。除空白对照组外均尾静脉注射C26结肠腺癌细胞单细胞悬液(密度:4×106/mL)0.1mL,形成结肠癌人工血行转移模型。按照小鼠灌胃容积0.2mL/10g分别予复方藤梨根制剂2.24g/10g、1.12g/10g、0.56g/10g、生理盐水灌胃。连续灌胃21天,第1、7、14、21天观察各组小鼠一般情况,异氟烷吸入式麻醉后,进行PET扫描,通过PET图像、靶区和非靶区比值(T/N)、标准化摄取值比值(SUV)数据分析,观察各组小鼠肿瘤生长情况。第21天处死小鼠,做肺组织病理活检。结果1模型组和复方藤梨根低剂量组小鼠恶液质症状较高、中剂量组小鼠明显;2各荷瘤组T/N值和SUV值较空白对照组明显升高(P<0.01),复方藤梨根制剂各组和模型组比较,肿瘤和非肿瘤区域T/N和SUV比值较低(P<0.01),高剂量组低于中、低剂量组,差异有统计学意义(P<0.05,P<0.01);3病理显示,各荷瘤组两肺均有转移灶,脑、肝脏、肾脏均未见明显肿瘤转移灶和癌前病变。结论经尾静脉接种C26结肠腺癌细胞可在Balb/c小鼠上形成稳定的肺转移模型,复方藤梨根制剂可以减少小鼠结肠癌人工肺转移模型的肿瘤活性,并抑制转移,有一定剂量依赖性。

Objective To investigate the effect of Fufang Tengligen mixture on pathophysiology, gross anatomy, molecular imaging, and pathology of the artificial blood metastasis from colon cancer in mice. Methods Fifty BALB/c mice were randomly divided into 5 groups：Fufang Tengligen high- , middle-, and low-dose groups（2.24g/10g, 1.12 g/10g, and 0.56g/10 g,respectively）, model group, and control group, 10 mice in each group. Except control group, other groups were intravenously injected with murine colon adenocarcinoma cells of 0.1 mL （4×106/mL） to make the artificial blood metastasis model.All mice in Fufang Tengligen mixture groups were given intra-gastrically with 0.2 mL/10 g medicine for 21 consecutive days. PET scanning was performed on day 1, 7, 14, and 21 after modeling. The T/N ratio, average SUV value of computer generated PET-CT images of the tumor were cal-culated for quantitive analysis. All mice were sacrificed on day 21 to perform lung biopsy. Results The symptom of cachexia was more obvious in model and Fufang Tengligen low-dose group than that in Fufang Tengligen high-and midddle-dose groups. The T/N ratio and SUV value were increased in tumor bearing groups than those in control group（P〈0.01）.Compared with model group, the T/N ratio and SUV value of the images of the tumor and non-tumor area were lower in Fufang Tengligen groups（P〈0.01）; the values were lower in Fufang Tengligen high-dose group than those in the middle-and low-dose groups（P〈0.05, P〈0.01）. Pathological examination showed metas-tasis in both lungs of tumor bearing groups, while no metastasis in brain, liver, and kidney was seen as well as precancerous lesions. Conclusion Fufang Tengligen mixture may have anti-tumor effect on lung cancer from colon cancer in mice in a dose-dependent manner.