夏天胶囊对硝酸甘油偏头痛模型大鼠行为及5-羟色胺含量的影响

Influence of Xiatian Capsule on Behavior and 5- hydroxytryptamine in Nitroglycerin Caused Migraine Model Rats

目的：观察夏天胶囊对硝酸甘油（GTN）偏头痛模型大鼠行为及5-羟色胺（5-HT）含量的影响。方法将60只大鼠随机分为6组，正常组、模型组、氟桂利嗪（0.5 mg/kg）组及夏天胶囊高、中、低剂量（相当于生药20，10，5 g/kg）组，连续灌胃给药7 d，末次给药后除正常组外所有大鼠均皮下注射GTN（10 mg/kg）复制偏头痛模型。观察大鼠在注射GTN 2~4 h内挠头次数，采用高效液相色谱法测定大鼠脑组织和血清5-HT含量。结果高、中剂量夏天胶囊能明显减少大鼠的挠头次数（ P＜0.01和 P＜0.05）；夏天胶囊高、中剂量组脑组织的5-HT含量较模型组显著升高（ P ＜0.01和 P ＜0.05），高剂量组血清的5-HT含量较模型组显著升高（ P ＜0.05）。结论夏天胶囊能有效缓解偏头痛的临床症状，还可有效升高模型鼠脑组织和血清5-HT的含量。提示其能有效治疗偏头痛的作用机制可能与升高脑组织和血清中5-HT的含量有关。

Objective To observe the influence of Xiatian Capsule on the behavior and 5-hydroxytryptamine（5-HT）content in nitro-glycerin（GTN）caused migraine model rats. Methods 60 rats were randomly divided into 6 groups：normal group,model group and flu-narizine（0. 5 mg/kg）group,high,middle and low dose（20,10,5 g crude drugs/kg）Xiatian Capsule groups. Gavage administration lasted for continuous 7 d. After the last time of gavage administration,all the rats except for the normal group were subcutaneously injected by GTN（10 mg/kg）for duplicating the migraine model. The times for scratching head within 2~4 h after the injection of GTN were ob-served. The high performance liquid chromatography（HPLC）method was adopted to detect the content of brain tissue and serum 5-HT. Results The high and middle dose Xiatian Capsule groups could significantly reduce the times of scratching head（ P 〈 0. 01,P 〈 0. 05）;the brain tissues 5-HT content in the high and middle dose Xiatian Capsule groups was increased significantly compared with the model group（ P 〈 0. 01,P 〈 0. 05）,the serum 5-HT content in the high dose Xiatian Capsule group was significantly increased com-pared with the model group（ P 〈 0. 05）. Conclusion Xiatian Capsule can effectively relieve the clinical symptoms of migraine,at the same time also can effectively increase the brain tissues and serum content of 5-HT in the model rats,which indicating that its mech-anism for effectively treating migraine may be related with the increases of the 5-HT content in brain tissue and serum.