高效液相色谱法测定蒙成药升阳十一味丸中没食子酸含量

Determination of Gallic Acid in Mongolian Patent Medicine Shengyang Shiyiwei Pills by HPLC

目的：建立测定蒙成药升阳十一味丸中没食子酸含量的高效液相色谱法。方法色谱柱为C18柱（250 mm ＆×4.6 mm，5μm），流动相为甲醇-0.1%磷酸水（5：95），检测波长为273 nm，流速为1.0 mL/min，柱温为30℃。结果没食子酸进样量在54.04~540.4 ng范围内与峰面积积分值线性关系良好（ r=1.0000），回归方程为 Y=12095 X-797509；平均加样回收率为99.50%，RSD为1.73%（ n=9）。结论该方法准确、精密、重复性好，可用于升阳十一味丸中没食子酸的含量测定。

Objective To establish the high performance liquid chromatography （ HPLC ） method of the gallic acid content in Mongolian patent medicine Shengyang Shiyiwei Pills. Methods The chromatographic separation was carried out on the Phenomen C18 column （250 mm ＆× 4. 6 mm,5 μm）with methanol-0. 1% phosphoric acid（5 ：95）as the mobile phase;the detection wavelength was set at 273 nm;the flow rate was 1. 0 mL/min and the column temperature was 30 ℃. Results The sample size of gallic acid in the concentration ranges of 54. 04-540. 4 ng showed good linear relation with the peak area （ r=1. 000 0）,the regression equation was Y=12 095 X-797 509;the average recovery rate was 99. 50%,RSD was 1. 73%（ n=9）. Conclusion The method is accurate and precise with good reproducibility,which can be used as the determination method of the gallic acid content in Mongolian patent medicine Shengyang Shiyiwei Pills.