喜炎平注射液溶血的分光光度法检查

The Spectrophotometric Method for the Hemolysis Examination of Xiyanping Injection

目的建立喜炎平注射液溶血检查的分光光度方法。方法按《中国药典》2010年版一部方法配制供试品溶液、阴性对照和阳性对照溶液,用分光光度法测定吸光度,并计算溶血率,检测波长545 nm。结果溶血率上限定为5%,样品的溶血率均<1%。结论喜炎平注射液可以用分光光度法进行溶血检查,其灵敏度高于药典方法。

Objective To establish a spectrophotometric method for the hemolysis examination of Xiyanping injection. Method The sample solution, negative control solution and positive control solution were prepared according to the method of Chinese Pharmacoperia （2010 edition, VolⅠ）, the light absorbance at the wavelength of 545 nm was detected using spectrophotometric method, and the hemolysis rate was calculated. Result The upper limit of the hemolysis rate was 5%, and the hemolysis rate of all of the samples was less than 1%. Conclusion The spectrophotometric method can be used for the hemolysis examination of Xiyanping injection, the sensitivity is higher than that of the Pharmacoperia method.