榄香烯诱导裸鼠移植瘤凋亡的作用机制研究

Th e Mechanism of Elemene Inducing Apoptosis of Nude Mice Transplanted Tumor

目的观察榄香烯对裸鼠胃癌移植瘤的作用并探讨其相关机制。方法雄性裸鼠建立人胃癌SCG-7901细胞裸鼠移植瘤模型,随机分为对照组、榄香烯组、PD98059组、榄香烯和PD98059联合组(榄香烯+PD98059组)。对比榄香烯和PD98059对裸鼠胃癌移植瘤的作用,测量对比裸鼠胃癌移植瘤的大小及药物对裸鼠生长的抑制作用。Western-blot方法检测各组ERK1/2蛋白、p-ERK1/2、p-P38蛋白的表达情况。结果 3个实验组在治疗后移植瘤大小与对照组比较明显减小,榄香烯组较对照组p-ERK1/2蛋白表达显著增多,3个治疗组相比对照组p-p38蛋白表达显著增加,两药联合比单一药物作用显著(均P<0.05或P<0.01)。结论榄香烯和PD98059都能促进肿瘤组织凋亡,其中榄香烯促进裸鼠胃癌移植瘤凋亡可能与p-ERK1/2蛋白和p-p38蛋白上调有关,而PD98059促进裸鼠胃癌移植瘤凋亡可能与p-P38蛋白上调有关。

Objective： To observe the effect of elemene inducing nude mice transplanted tumor and to explore the relative mechanism. Method： male nude mice were chosen to establish SCG-7901 gastric transplanted tumor model and were divided into four groups：control group,elemene group （maximum tolerated dose of 200 mg/kg）, PD98059 group（maximum tolerated dose 1 mg/kg） ,elemene and PD98059 joint group. The effect of elemene and PD98059 on nude mice gastric transplanted tumor were compared,and the size of transplanted tumor were mea- sured and the inhibitive effect on the growth of nude mice were determined. ERK1/2,P-ERK1/2 and P-P38 pro- tein expressions of each group were detected by Western blot method. Result： The size of nude mice transplanted tumors of three experimental groups significantly decreased after treatment compared with control group. And P- ERK1/2 protein expression of elemene group was more obvious than that of the control group. P-P38 protein ex- pression of three treatment groups increased much more significantly than that of the control group. Elemene and PD98059 combined treatment group had more obvious effect than single drug group. Conclusion： Both elemene and PD98059 can promote tumor tissue apoptosis. Elemene promoting nude mice gastric transplanted tumor apop- tosis may be related to increased P-ERK1/2 protein and P- P38 protein,while PD98059 promoting such apoptosis may be related to increased P- P38 protein.