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泛素连接酶Nedd4调控人前列腺癌细胞的增殖与凋亡

Effects of Ubiquitin Ligase Nedd4 on Human Prostate Cancer Cell Proliferation and Apoptosis
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摘要 为了研究泛素连接酶Nedd4对肿瘤细胞增殖和凋亡的影响,利用小RNA干扰技术,通过慢病毒包装,侵染前列腺癌细胞DU145,达到下调Nedd4表达的目的.MTT检测表明,下调Nedd4表达可以抑制DU145细胞的增殖;DAPI染色发现,下调Nedd4表达的DU145细胞对星形孢菌素诱导的细胞凋亡更加敏感.这些结果都说明,下调Nedd4的表达不仅可以抑制DU145肿瘤细胞增殖,而且可以促进细胞凋亡. To study the effects of Nedd4 on tumor cell proliferation and apoptosis,Nedd4 was depleted in prostate cancer cell DU145 by infecting the lentiviruses expression of Nedd4-shRNAs. MTT assay showed that that depletion of Nedd4 de-creased DU145 cell proliferation. DAPI staining indicated that knockdown of Nedd4 resulted in greater sensitivity to stauro-sporine-induced apoptosis of DU145 cells. These results revealed that the down-regulation of Nedd4 expression not only reduced the cell proliferation but also induced the apoptosis of DU145 cells.
作者 周洁 李玉银 赵昌彩 刁爱坡
机构地区 天津科技大学生物工程学院
出处 《天津科技大学学报》 CAS 2014年第5期15-19,共5页 Journal of Tianjin University of Science & Technology
基金 天津市自然科学基金重点资助项目(10JCZDJC16800) 国家自然科学基金资助项目(31071181)
关键词 Nedd4 前列腺癌细胞 细胞增殖 细胞凋亡 Nedd4 prostate cancer cell cell proliferation cell apoptosis
分类号 R737.25 [医药卫生—肿瘤]
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参考文献27

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二级参考文献14

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天津科技大学学报
2014年 第5期

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