摘要
目的检测Syn、Bax、Bcl-2蛋白在帕金森病大鼠模型结肠中的表达及意义,探讨帕金森病消化系统功能紊乱的可能机制。方法采用羟多巴胺(6-hydroxydopamine,6-OHDA)损毁大鼠中枢黑质多巴胺能神经元,诱导制备实验性帕金森病大鼠模型。应用免疫组化法S-P法检测Syn、Bax、Bcl-2在帕金森病模型大鼠及正常对照组大鼠结肠组织中的表达情况。结果造模成功后,用免疫组化法S-P法,发现帕金森模型大鼠结肠中的Syn和Bax表达水平较对照组增加,而Bcl-2表达水平较对照组减少。结论帕金森大鼠结肠组织中的Syn及Bax表达增加,Bcl-2表达减少,为研究帕金森患者肠道功能紊乱的机制提供线索。
Objective To detect the expression and significance of Syn,Bax,Bcl 2 in colon of rat model of Parkinson＇s disease,and to explore the possible mechanism of digestive system disorder of Parkinson＇s disease. Methods 6-hydroxydopamine （6-OHDA） damaged rat central dopaminergic neurons in the substantia nigra were used to prepare experimental rat model of Parkinson＇s disease. S P method of immunohistochemical was applied to detect the expression of Syn, Bax, Bcl-2 of rats＇ colon tissue in rat model of Parkinsons disease and normal control group. Results After the success of the model,compared with the control group, Syn and Bax expression levels increased in colon of rat model of Parkinson＇s disease by immunohistochemical S-P method,while the Bcl-2 expression level was reduced. Conclusion The expressions of Syn and Bax in colon tissue of rat model of Parkinson＇s disease increase,but the expression of Bcl 2 reduces,which provide clues to research the mechanism of intestinal dysfunction in Parkinson＇s disease.
出处
《中国实用神经疾病杂志》
2014年第19期10-11,共2页
Chinese Journal of Practical Nervous Diseases
基金
2014年唐山市科学技术研究与发展指导计划(项目编号:13130292a)
