原子荧光光度法测定生物材料中总汞

Determination of total mercury in biological materials by atomic fluorescence spectrometry

目的 建立应用PF6-2型双道原子荧光光度计测定生物材料中总汞的方法。方法 样品经湿法或微波消解后,用原子荧光光度法测定其汞含量。结果 检出限为0.11 ng/ml,在0-10 mg/ml内线性关系良好,相关系数r为0.999 7。与现行测定汞的方法相比,测定结果差异无统计学意义（P〉0.05）。微波消解的精密度相对较好,加标回收率在91.0%-104.0%之间。结论 原子荧光光度法分析速度快,检出限低,精密度和准确度好,适合生物材料中总汞的测定。

Objective To establish a method for measuring total mercury in biological materials with double channel atomic fluorescence spectrometer of type PF6 - 2. Methods Mercury in the sample was measured with atomic fluorescence spectrometry after wet digestion or microwave digestion processes. Results The detection limit was 0. 11 ng/ml with a good linear relationship between 0 - 10 mg/ml, the correlation coefficient was 0. 999 7. There was no significant difference （ P 〉 0. 05 ） in results of the determination between this method and the current method for measuring mercury. The precision of microwave digestion was better. The recovery of standard addition was 91.0% - 104. 0%. Conclusion The speed of analysis of atomic fluorescence spectrometry is fast, accuracy and high precision, and it is a good method for the determination of total mercury in biological materials.