离子色谱法测定盐酸托烷司琼中的三氟乙酸

Determination of trifluoroacetic acid in tropisetron hydrochloride by ion chromatography

目的：建立离子色谱检测盐酸托烷司琼中三氟乙酸的方法。方法采用Ionpac AS11-HC色谱柱（4 mm ×250 mm）以及相应的保护柱Ionpac AG11-HC（4 mm ×50 mm），淋洗液为含有4.5 mmol·L-1 Na2 CO3和0.8 mmol·L-1 NaHCO3的溶液，流速为1.2 mL·min-1，抑制电导检测，电导池温度：35℃，柱温：35℃，进样量：25μL。结果三氟乙酸和样品中其他成分能达到很好的分离；在0.2613~8.7113 μg·mL-1范围内具有很好的线性关系（ r=0.9999），检出限为0.0862μg·mL-1，定量限为0.2613μg·mL-1。结论该方法专属性强，灵敏度高，可作为盐酸托烷司琼中三氟乙酸的检测有效方法。

Objective To develop an ion chromatography method for the determination of trifluoroacetic acid in tropise-tron hydrochloride. MetHods The method uses an Ionpac AS11-HC column（4 mm × 250 mm）and a guard column（4 mm × 50 mm）,taking mixture of 4. 5 mmol·L-1 sodium carbonate and 0. 8 mmol·L-1 sodium hydrogen carbonate solution as eluent at a flow rate of 1. 2 mL·min-1 . The cell heater temperature was 35℃,column temperature was 35℃ and injection volume was 25 μL. Results There was a good resolution between trifluoroacetic acid and other components. The linear range of trifluoroacetic acid was 0. 261 3~8. 711 3 μg·mL-1（r=0. 999 9）. LOD was 0. 086 2 μg·mL-1,and LOQ was 0. 261 3 μg·mL-1. CoNclusioN This method had good specificity and sensitivity. It can be used to detect trifluoroacetic acid in tropisetron hydrochloride.