CX37基因1019C/T多态性与腰椎间盘突出症及临床分型的关系

Relationship between 1019C /T polymorphism of CX37 gene and clinical classification of lubar intervertebral disc protrusion

目的探讨间隙性连接蛋白37基因1019C/T多态性位点与腰椎间盘突出症及临床分型的关系。方法采用基因测序技术,检测腰椎间盘突出症组191例(根据影像学表现分为A组:膨出型64例,B组:突出型98例,C组:脱出型29例)和健康对照组187例CX37基因1019C/T多态性分布。结果腰椎间盘突出症组和对照组均存在CX37基因1019C/T多态性,2组均以C等位基因为主。突出型组、脱出型组的CC基因型和C等位基因(CC+CT)分布频率明显高于对照组(P<0.05)。在性别亚分组分析比较中,男性人群的CC基因型和C等位基因频率在突出型组和脱出型组中明显高于对照组,而女性在3组中均未发现上述明显差异。另外在膨出型组和对照组基因型、等位基因频率分析比较中未发现差异有统计学意义。结论 CX37等位基因可能与腰椎间盘突出症相关。

Objective To investigate the relationship between 1019C/T polymorphism of CX 37 gene and Clinical classification of lubar intervertebral disc protrusion. Methods Using the gene sequencing technology, to determining a total of 191 cases were diagnosed of lumbar disc herniation （According to the imaging findings divide into group A：bulging type 64 cases, group B： highlight type 98 cases and group C： prolapsed-type 29 cases）, and 187 healthy people served as control group. All cases were genotyped by DNA sequencing.Results A polymorphism C1019T on the Connexin37 gene was found in the whole population and the distributions of three genotype frequencies in both groups were in accordance with the Hardy-Weinberg equilibrium. And the two groups are mainly C allele carries. The genotype frequencies of CC and C allele carries in connexin37 C1019T polymorphism in Prominent type group was significantly higher than control group （ P 〈 0.05 ）. And the genotype frequencies of CC and C allele carries in connexin37 C1019T polymorphism in extrusion-type group was significant-ly higher than control group,too （ P 〈 0.05）. In the sub-group analysis and comparison of gender,the frequency of CC genotype and C allele frequencies in male population is higher than control group. The female group and bulging group were not found significant differences in the three groups. Besides, in the bulging type group and genotype of control group, allele frequency analysis comparison were not found significant differences. Conclusion The C allele in the CX37 gene might be associated with the susceptibility to LDH.