三种金花茶叶的薄层色谱鉴别研究

Studies on Idetification of three varieties Camellia by Thin-Layer Chromatography

目的:建立金花茶叶的薄层色谱鉴别方法。方法:采用薄层色谱法分别鉴别金花茶叶中的齐墩果酸、芦丁和人参皂苷Rg1等化学成分。结果:齐墩果酸、芦丁和人参皂苷Rg1分别在以石油醚-乙酸乙酯-丙酮(7∶2∶1)、乙酸乙酯-甲醇-水-甲酸(9∶2∶1∶0.1)和氯仿-乙酸乙酯-甲醇-水(3∶8∶4.4∶1.6)为展开剂的条件下获得理想分离,且在同一点样量和展开条件下,三个金花茶品种中的齐墩果酸、芦丁、人参皂苷Rg1斑点均有明显差异。结论:该方法简便、快速,可更好地控制金花茶叶的药材质量。

Objective To establish a method for identification of Camellia by Thin-Layer Chromatography （TLC）.Methods The specific compouds,oleanolic acid,rutin and ginsenosides Rg1 were studied by TLC in three different Camellia species.Results These three kinds of ingredients were all separated very well by the Ligroin-acetoacetate -acetone （7∶2∶1 ）,Acetoacetate -methanol-aquaefer-formic acid （9∶2∶1∶0.1 ）,Chloroform-acetoacetate-methanol-aquaefer （3∶8∶4.4∶1.6 ）,and under the same condi-tions,the spots of oleanolic acid,rutin and ginsenosides Rg1 were significantly in different three varieties of Camellia.Conclusion This method was simple ,quick,and can better control the quality of the Camellia.