HPLC法测定百部止咳糖浆中苦杏仁苷的含量

Determination of the content of amygdalin Baibu Zhike syrup by HPLC

目的：建立百部止咳糖浆中苦杏仁苷的含量测定方法。方法：应用HPLC检测法，色谱柱为Kromasi C18（5μm，4.6mm ×250mm），流动相为甲醇-水（20∶80），柱温25℃，流速1.0ml/min。检测波长215nm。结果：苦杏仁苷在0.04～0.24mg/ml范围内线性关系良好（r＝0.9995），平均回收率99.65％。RSD为1.90％。结论：该方法准确、快速、可靠，可用于测定百部止咳糖浆中苦杏仁苷的含量。

Objective To establish a method for determination of amygdalin Baibu Zhike syrup. Methods Using HPLC method, the column was KromasiC18 （5μm,4.6mm ×250mm）,mobile phase of methanol -water （20∶80）,the column temperature was 25℃,flow rate of 1.0ml/min.The detection wavelength was 215nm.Results The amygdalin in the range of 0.04-0.24mg/ml good linear relationship （r=0.9995 ）,the average recovery was 99.65%. RSD 1.90%.Conclusion The method is accurate,fast,relia-ble,and can be used for content determination of amygdalin Baibu Zhike syrup.