大鼠恐惧心理应激模型—模拟地震法建立及评价

Establishment and evaluation of earthquake simulation method in fear stress experiment with rats

目的在恐惧心理应激实验中检测自制模拟地震法的实效性。方法实验设对照组、旁观电击组和模拟地震组,其中模型组分别采用自制模拟地震法及经典的旁观电击法制备恐伤母鼠动物模型,通过观察3组母鼠孕产情况及1月龄仔鼠认知记忆能力评判模型建立是否成功。结果与对照组比较,模拟地震及旁观电击组母鼠产仔数减少,差异均有统计学意义(P<0.05);与对照组比较,旁观电击组仔鼠4d平均逃避潜伏期[分别为(89.5±27.2)、(70.7±40.0)、(57.4±38.3)、(55.5±35.9)s]均延长,差异有统计学意义(P<0.05),与旁观电击组比较,模拟地震组仔鼠平均逃避潜伏期均缩短,差异有统计学意义(P<0.05);与对照组比较,旁观电击组仔鼠平均游泳速度变慢(P<0.05),在20%边缘区平均百分比增大(P<0.05),在其他区域平均百分比减小(P<0.05),差异均有统计学意义(P<0.05),模拟地震组仔鼠平均游泳速度减慢,差异有统计学意义(P<0.05);模拟地震组与旁观电击组比较,各指标差异均无统计学意义(P>0.05)。结论模拟地震法应用到恐惧心理应激实验中具有一定的可行性,但需进一步改进完善。

Objective To evaluate the effectiveness of the earthquake simulation in fear stress experiment with rats.Methods Forty-five Wistar pregnant rats were divided into a control group and two model groups（communication box and earthquake simulation group）.The two model groups were established by making the rats watching other rats shocked by electricity and simulating earthquake and the models were evaluated by observing the number of filial rats and the cognitive ability of the offspring at the age of 1 month.Results Compared with the control group,the number of filial rats in two model groups was reduced,with significant differences（P〈0.05）.Compared with the control group,the average escape latency periods were 89.5±27.2,70.7±40.0,57.4±38.3,and55.5±35.9 s for the rats in communication box group on the first to 4th day of the experiment,with significant differences（P〈0.05）.Compared with the communication box group,the average escape latency periods were shortened in the earthquake simulation group,with a significant difference（P〉0.05）.In spatial probe test,compared with the control group,the average swimming speed of the communication box group was slower（P〈0.05）and the percent of the 20% marginal area increased（P〈0.05）,but decreased for other areas（P〈0.05）,with significant differences（P 〈 0.05）;the average swimming speed of earthquake simulation group was slower（P〈0.05）,with a significant difference（P〈0.05）.Conclusion The earthquake simulation method is feasible for fear stress experiment with rats but needs to be improved.