再生丝素/明胶-聚己内酯皮芯结构纳米纤维膜的制备及生物性能研究

Study on Preparation and Biological Properties of Nanofiber Membrane with Regenerated Silk Fibroin/Gelatin-Polycaprolactone Skin-core Structure

利用同轴静电纺丝技术,制备以丝素(SF)/明胶(GE)共混物为皮层,聚己内酯(PCL)为芯层的复合纳米纤维膜。研究不同芯层浓度对复合纳米纤维膜形貌、孔径、力学性能和生物相容性的影响。结果表明:所制备的SF/GE-PCL复合纳米纤维表面光滑且有明显的皮芯结构,随着芯层浓度由4%增大到10%,复合纳米纤维的平均直径从256nm增大到941nm,纤维膜的平均孔径从0.576μm增大到1.018μm,纤维膜的断裂强度和断裂应变增大,人皮肤成纤维细胞能在皮芯结构纳米纤维膜上黏附、生长和增殖。

This study prepares composite nanofiber membrane with silk fibroin（SF）/gelatin（GE）blend as skin layer and polycaprolactone（PCL）as core layer through coaxial electro-spinning technology and studies the influence of different core layer concentrations on the morphology,bore diameter,mechanical properties and biological compatibility of composite nanofiber membrane.The result shows that the SF-GE-PCL composite nanofiber prepared has a smooth surface and obvious skin-core structure;as core layer concentration increases from 4%to 10%,the average diameter of composite nanofiber increases from256 nm to 941 nm,the average bore diameter of fiber membrane increases from 0.576μm to 1.018μm and the breaking strength and breaking strain of fiber membrane increase;fibroblast of human skin can adhere to and grow and proliferate on nanofiber membrane with skin-core structure.