小麦GDH1基因克隆及其功能标记开发

Cloning and Functional Marker of GDH1 Gene in Wheat

谷氨酸脱氢酶（glutamate dehydrogenase,GDH）在植物体内催化合成谷氨酸的可逆反应,通过GDH固氮比谷氨酸合成酶途径更节省能量。在植物大多数组织中,GDH1是该基因家族中表达最高的基因,比其他GDH成员具有更为重要的作用。本研究从普通小麦基因组中分离了TaGDH1基因在A、B、D染色体组的序列。针对TaGDH1a基因在基因组DNA序列1 900-1 983 bp位置存在的核苷酸差异设计了一个插入/缺失标记,同时将该标记定位在5A染色体上。

Glutamate dehydrogenase （ GDH ） is an enzyme catalyzing a reversible reaction of compounding L-glutamate.Assimilation of ammonia via GDH is more energy -efficient than that via glutamate synthase .GDH1 gene was the dominant form in most of examined tissues , perhaps suggesting its greater functional significance than other GDH genes.In this study, the genomic sequence on chromosome A , B and D of a no-vel gene, designated TaGDH1, was isolated from the hexaploid wheat （ Triticum aestivum L.） genome databases .An InDel marker was designed based on the insert /deletion divergence in the 1 900-1 983 bp position of TaGDH1a gene sequence, and was located on the chromosome 5A in wheat.