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bar基因表达蛋白单克隆抗体制备及ELISA检测方法的建立 被引量：2

Development of A Monoclonal Antibody Sandwich ELISA for Detection of bar Gene Transgenic Plant and Element
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摘要 通过原核表达诱导bar基因表达蛋白,通过表达蛋白免疫BALB/c小鼠,经细胞融合、筛选克隆,获得一个杂交瘤细胞株。以兔多抗作为捕获抗体,鼠单抗作为示踪抗体,辣根过氧化物酶标记羊抗鼠IgG作为检测系统,建立了转基因植物bar基因检测双抗夹心ELISA方法,试验证明利用该抗体建立的ELISA检测方法含有转bar基因的转基因玉米成分具较好的特异性和灵敏度。为转基因成分的检测提供了安全、快速、准确的技术手段。 This study induced prokaryotic express Bar protein, by protein Immunity of BALB/c mice, cell fusion and screening clones, we obtained a hybridoma cell line. In this study, using rabbit polyclonal IgG and hybridoma cells secreting monoclonal antibodies we established sandwich ELISA method for detection of bar gene intransgenic plant which showed good specificity and sensitivity . This study provides rapid and accurate.technical tool for a secure monitoring of GMO.
作者 龙丽坤 李葱葱 张明 李飞武
机构地区 吉林省农业科学院
出处 《生物技术通报》 CAS CSCD 北大核心 2014年第10期71-75,共5页 Biotechnology Bulletin
基金 吉林省人力资源与社保厅项目博士后启动项目
关键词 BAR基因 杂交瘤细胞 单克隆抗体 转基因检测 bar gene Hybridoma Monoclonal antibody GMO detection
分类号 Q78 [生物学—分子生物学]
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  • 1Sisi Kang,Mei Yang,Zhongsi Hong,Liping Zhang,Zhaoxia Huang,Xiaoxue Chen,Suhua He,Ziliang Zhou,Zhechong Zhou,Qiuyue Chen,Yan Yan,Changsheng Zhang,Hong Shan,Shoudeng Chen.Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites[J].Acta Pharmaceutica Sinica B,2020,10(7):1228-1238. 被引量：25
  • 2许文涛,黄昆仑,邓爱科,罗云波.转基因产品中PAT蛋白的酶联免疫检测(英文)[J].农业生物技术学报,2006,14(2):250-254. 被引量：7
  • 3刘洪艳,弭晓菊,崔继哲.bar基因、PAT蛋白和草丁膦的特性与安全性[J].生态学杂志,2007,26(6):938-942. 被引量：27
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生物技术通报
2014年 第10期

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