高效氯氰菊酯对斑马鱼胚胎毒性的研究

Toxicity of Beta-cypermethrin for Zebrafish Embryos

高效氯氰菊酯是一种Ⅱ型拟除虫菊酯类农药,对鱼类及其它水生生物具有高毒,且在其它食物中也可检测到它的残留,高效氯氰菊酯的残留对人类的健康同样具有不容忽视的影响。为了解高效氯氰菊酯对鱼、对人类的毒性机制,选用0.05、0.1、0.2、0.6、1 mg/L五个梯度浓度的高效氯氰菊酯溶液,对斑马鱼胚胎进行毒性处理,在显微镜下观察其对斑马鱼发育形态变化和孵化率等影响。发现高效氯氰菊酯农药对斑马鱼胚胎有严重的致畸效应,这种致畸性呈现剂量依赖性。使用0.1 mol/L浓度的高效氯氰菊酯农药对斑马鱼胚胎进行处理,进行早期胚胎发育的毒性研究。采用荧光差异双向凝胶电泳(2-D DIGE)-质谱相结合的方法,鉴定出11个差异表达蛋白质。这些蛋白质与细胞骨架、应激反应和新陈代谢有关。这些研究结果为进一步研究高效氯氰菊酯对动物的毒性机制提供了基本信息。

Beta-cypermethrin, an insecticide of type II pyrethroids, is highly toxic to fish and other aquatic organism and the residues can be detected in many foods. The impact of beta-cypennethrin for human health can not be neglected. To bettgr define the toxicity mechanism of beta-eypermethrin for fish and human, the embryos of zebrafish were exposed to different sub-lethal concentrations of 0.05, 0.1, 0.2, 0.6 and 1 mg/L of beta-CYP solutions. With the help of a microscope, we observed the morphological changes. The results displayed that exposed to beta- CYP caused the embryonic toxicity increased in a close-dependent manner. We chose zebrafish embryos exposure to beta-CYP （ 0.1 mol/L ） as a model system to investigate the toxic effects on early development. A two-dimensional difference gel eleetrophoresis （ 2-D DIGE ） coupled with MALDI-TOF-MS/MS analysis was employed to detect and identify the differential expressed proteins. In the present study, a total of 11 different proteins were successfully identified, which were mostly associated with cytoskeletal, stress response and metabolism. This study brings useful information and contributes to a better understanding about the embryo toxicity organisms of beta-CYP.