P-选择素蛋白及mRNA在宫颈鳞癌中的表达及生物学行为的研究

Study on the expressions of P-selectin protein and mRNA in cervical squamous cell carcinoma and the biological behavior

目的：检测宫颈鳞癌组织中P-选择素蛋白及mRNA的表达情况,探讨其在宫颈鳞癌发生发展中的作用。方法：选取2011年1月～2014年4月承德市多家医院手术切除的宫颈鳞癌标本60例为病例组,宫颈正常组织40例为对照组。采用免疫组化方法与逆转录-聚合酶链式反应（RT-PCR）技术联合检测两组宫颈组织中P-选择素蛋白及mRNA的表达情况。结果：病例组P-选择素蛋白及mRNA的表达阳性率（63.3%和58.3%）均高于对照组（35.0%和27.5%）,差异有统计学意义（P〈0.05）;病例组P-选择素蛋白及mRNA的表达均与分化程度、p TNM分期、淋巴结转移和浸润深度相关（P〈0.05）,而与年龄无关（P〉0.05）。结论：宫颈鳞癌组织中P-选择素蛋白及mRNA的表达与宫颈鳞癌的发生、发展紧密相关。应用免疫组化方法与RT-PCR技术联合检测宫颈鳞癌组织中P-选择素蛋白及mRNA可评估宫颈鳞癌浸润和转移的能力,并为预后的预测提供理论依据。

Objective： To detect the expressions of P - selectin protein and mRNA in cervical squamous cell carcinoma, explore its role in occurrence and development of cervical squamous cell carcinoma. Methods： Sixty cervical squamous cell carcinoma samples from many hospitals in Chengde city from January 2011 to April 2014 were selected as case group, 40 normal cervical samples were selected as control group. Immunohistochemical SP method and RT - PCR were used to detect the expressions of P - selectin protein and mRNA in the two groups. Results： The positive expression rates of P - selectin protein and mRNA in case group were 63.3% and 58. 3 % , respectively, which were statistically significantly higher than those in control group （35.0% and 27. 5% ） （P 〈0. 05） ; the expressions of P - selectin protein and mRNA in case group were correlated with differentiated degree, TNM classification, lymph node metastasis and depth of invasion （ P 〈 0.05 ） , which were not correlated with age （ P 〉 0. 05 ） . Conclusion： The expressions of P - selectin protein and mRNA in cervical squamous cell carcinoma are closely correlated with growth and development of cervical squamous cell carcinoma. Immunohistochemistry and RT - PCR combined with joint detection of P - seleetin protein and mRNA in cervical squamous cell carcinoma can evaluate invasion and me- tastasis of cervical squamous cell carcinoma, which can provide a theoretical basis for predicting prognosis.