IEX-1、HIF-1α和NF-κB蛋白在宫颈癌组织中的表达及临床意义

Expressions of IEX-1,HIF-1α and NF-κB in cervical cancer and their significance

目的：通过检测IEX-1、HIF-1α和NF-κB蛋白在宫颈癌组织和细胞中的表达情况,初步探讨其与宫颈癌发病的关系。方法：选取2009年1月~2011年10月该院病理科手术存档的蜡块标本116份,根据病变程度分为宫颈癌患者标本50份（A组）、宫颈上皮内瘤变（CIN）患者标本40份（B组）和正常宫颈标本26份（C组）。采用免疫组化法检测3组宫颈组织中IEX-1、HIF-1α和NF-κB蛋白的表达,并分析3者的相关性。结果：IEX-1在A组的阳性表达率明显低于B组和C组,差异有统计学意义（P〈0.05）,HIF-1α和NF-κB在A组的阳性表达率均明显高于B组和C组,差异有统计学意义（P〈0.05）;A组IEX-1、HIF-1α和NF-κB的表达均与组织学分级及有无淋巴结转移相关（P〈0.05）,与患者年龄、肿瘤的组织学类型和FIGO分期无关（P〉0.05）;A组HIF-1α和NF-κB的表达呈正相关（r=0.398,P〈0.05）,两者与IEX-1的表达呈负相关（r=-0.322、-0.517,P均〈0.05）。结论：IEX-1、HIF-1α和NF-κB蛋白的表达变化与宫颈癌的发生发展有关。

Objective： To explore the relationship between IEX - 1, HIF - 1α, NF - kB and oncogenesis of cervical cancer by de- tecting the expressions of 1EX - 1, HIF - 1α and NF - KB in cervical cancer tissue and cells. Methods： A total of 116 paraffin - embedded cervical tissue specimens were selected from Department of Pathology in the hospital from January 2009 to October 2011, then they were divided into group A （50 cervical cancer specimens）, group B （40 cervical intraepithelial neoplasia specimens） and group C （26 normal cer- vical specimens） according to degree of the disease. Immunohistochemical method was used to detect the expressions of IEX - 1, HIF - 1α and NF - kB in the three groups, the correlation was analyzed. Results： The positive expression rate of IEX - 1 in group A was statistically significantly lower than those in group B and group C （P 〈 0.05 ）, the positive expression rates of HIF -1α and NF - kB in group A was sta- tistically significantly higher than those in group B and group C （P 〈 O. 05） . In group A, the expressions of IEX - 1, HIF -1α and NF -kB were correlated with histological grades and lymph node metastasis （P 〈 0. 05 ）, but the expressions of IEX- 1, HIF-1α and NF-kB were not correlated with age, histological types and FIGO staging （P 〉 0.05 ） ; there was a positive correlation between HIF -1α expression and NF - kB expression in group A （ r = 0. 398, P 〈 0. 05 ）, there was a negative correlation between HIF - 1 a expression, NF - kB expression and IEX - 1 expression （ r = - O. 322, P 〈 O. 05 ; r = - O. 517, P 〈 O. 05 ） . Conclusion ： The changes of expressions of IEX - 1, HIF - 1α and NF - kB are correlated with oncogenesis and development of cervical cancer.