电针对抑郁大鼠海马E-选择素和抵抗素蛋白表达的影响

Influence of Electro-acupuncture on E-Selectin and Resistin Protein Expression in Hippocampus of Rats with Chronic Stress Depression

目的观察电针对慢性应激抑郁模型大鼠海马E-选择素和抵抗素蛋白表达的影响,探讨电针对抑郁症脑内微环境的作用机制。方法采用慢性应激结合孤养方法造模。将40只SD大鼠随机分为空白组、模型组、电针组、氟西汀组,每组10只。于每日造模前1 h,电针干预"百会"、"印堂",频率2 Hz,电流强度0.6 mA,每次20 min;氟西汀灌胃干预,用药剂量10 mg/kg,给药容积5 mL/kg。采用生物素标记抗体芯片技术检测大鼠海马E-选择素和抵抗素蛋白表达水平。结果与空白组比较,模型组大鼠海马E-选择素和抵抗素蛋白水平上调(fold change=1.23,1.22);与模型组比较,电针组、氟西汀组E-选择素水平下调(fold change=0.65,0.62)、抵抗素表达水平下调(fold change=0.76,0.65)。结论电针干预可下调慢性应激抑郁模型大鼠海马E-选择素和抵抗素蛋白表达。

Objective To observe the effects of electro-acupuncture on the E-selectin and resistin protein expression in the hippocampus of rats of chronic stress depression;To explore the role and mechanism of electro-acupuncture in the micro-environment of brain with depression. Methods 40 Fourty rats were randomly divided into four groups：blank group, model group, model＋EA group, and model＋fluoxetine group by using chronic stress combined with solitary raising methods, 10 rats in each group. Electro-acupuncture intervention was used 1 hour before the modeling. Electro-acupuncture was given at points Baihui （Du 20） and Yintang （Extra） for twenty minutes （2 Hz, 0.6 mA） every day. Fluoxetine was given at the volume of 5 mL/kg, dosage of 10 mg/kg. E-selectin and resistin protein expression in hippocampus were determined by biotin label-based antibody array. Results Compared with the blank group, the protein expression of E-selectin and resistin in the model group increased （fold change=1.23, 1.22）. Compared with the model group, E-selectin and resistin expression decreased （fold change=0.65, 0.62;fold change=0.76, 0.65）. Conclusion Electro-acupuncture intervention could down-regulate E-selectin and resistin expression in the hippocampus of chronic stress depression model.