HPLC同时测定三味甘露散中土木香内酯和异土木香内酯含量

Simultaneous Content Determination of Alantolactone and Isoalantolactone of Radix Inulae in Sanwei Ganlusan by HPLC

目的：建立同时测定三味甘露散中土木香内酯、异土木香内酯含量的方法。方法采用高效液相色谱法,光电二极管阵列检测器,色谱柱为 CAPCELL PAK MG C18（4.6 mm×250 mm,5μm）,流动相为乙腈-0.4%磷酸（58∶42）,流速1.0 mL/min,检测波长225 nm,柱温30℃。结果土木香内酯在0.083～0.517μg、异土木香内酯在0.108～0.672μg 范围内呈良好线性关系,r＝0.9999；土木香内酯平均加样回收率为97.95%,RSD＝1.31%；异土木香内酯平均加样回收率为97.69%,RSD＝1.24%。结论本方法操作简便,可快速、准确地测定三味甘露散中土木香内酯和异土木香内酯的含量。

Objective To establish a method to simultaneously determine contents of alantolactone and isoalantolactone in Sanwei Ganlusan. Methods Contents of alantolactone and isoalantolactone was determined by HPLC was used with PDAD detector;the column was CAPCELL PAK MG C18 （4.6 mm×250 mm, 5μm）;the mobile phase consisted of acetonitrile-0.4%phosphoric （58∶42）;the flow rate was 1.0 mL/min;the detection wavelength was set at 225 nm;the column temperature was maintained at 30 ℃. Results The linear range of alantolactone was 0.083-0.517 μg （r=0.999 9）, and isoalantolactone was 0.108-0.672 μg （r=0.999 9）. The mean recovery of alantolactone was 97.95%, RSD=1.31%. The mean recovery of isoalantolactone was 97.69%, RSD=1.24%. Conclusion The method is accurate and simple in operation, which can be used to simultaneously determine contents of alantolactone and isoalantolactone in Sanwei Ganlusan.