摘要
目的 建立HPLC法对金芪胶囊中芍药苷的含量测定方法.方法 选择Hypersil ODS柱(200 mm×4.6 mm,5μm);乙腈-0.1%磷酸溶液(12∶88)为流动相;流速1.0mL/min;检测波长230nm,柱温为30℃.结果 金芪胶囊中芍药苷的含量在0.31~2.45μg的范围内,有良好的线性关系,r=0.9996,平均回收率为99.33%,稳定性(RSD)=0.14%,金芪胶囊中芍药苷的含量为0.5897mg/g.结论 该方法操作易行,稳定可靠,可用于金芪胶囊的质量控制.
Objective To establish a method for content determination of paeoniflorin in HPCL produced Jinqi capsule.Metheds Column Hypersil ODS column (200 mm×4.6 mm,5 μm );mobile phase, acetonitrile phosphoric acid solution (12 : 88); flow rate= 1.0 mL/min; detection wavelength :230 nm,the column temperature was 30℃.Results The content of paeoniflorin Jinqi Capsules in the range of 0.31 to 2.45 μg showed a linear relationship,good r=0.9996,the average recovery was 99.33%, RSD = 0. 14%,and the content of paeoniflorin Jinqi Capsules was 0.5897mg/g.Cenclusien Being easy, stable and reliable,The method can be used for quality control of Jinqi capsules.
出处
《湖北民族学院学报(医学版)》
2014年第3期35-36,39,共3页
Journal of Hubei Minzu University(Medical Edition)