催化光度法测定铜(Ⅱ)的研究

Study of detecting Cu(Ⅱ)by catalytic spectrophotometer

根据铜（Ⅱ）能催化溴酸钾氧化靛蓝胭脂红的褪色反应,建立一种快速简捷的测定铜（Ⅱ）的新方法.优化试验条件：最大吸收波长、氧化剂及显色剂用量、活化剂用量、缓冲溶液酸度及用量、反应温度、反应时间.在选定的最佳试验条件下,当铜（Ⅱ）浓度在0-10μg/25mL范围时,ΔA与铜（Ⅱ）浓度呈良好的线性关系.其回归方程为：ΔA=0.014C＋0.0011,相关系数r=0.9996,检测限为0.0086μg/mL,加标回收率为97.4%-100.4%.

According to the fading reaction that copper（II）can catalyze potassium bromate oxidize indigo carmine,a rapid and simple new method to determination of copper（II）is established.Optimization of experimental conditions：the maximum absorption wavelength,oxidizing agent and dosage of chromogenic agent,activating agent dosage,buffer solution acidity and dosage,reaction temperature,reaction time.In the conditions selected,when the copper（II）at the concentration of 0-10μg/25 mL,ΔAand the copper（II）concentration shows a good linear relationship.The regression equation is：ΔA=0.014 C ＋0.0011,the correlation coefficient r=0.9996,the detection limit is 0.0086μg/mL,and the recovery is97.4%-100.4%.