兰州地区腹泻患者艰难梭菌的感染状况调查

Investigation on Clostridium difficile infection among diarrhea patients in Lanzhou area

目的调查兰州地区腹泻患者中艰难梭菌的流行特点,揭示国内艰难梭菌感染的现况。方法通过细胞毒检测试验和酶联免疫吸附试验对206份临床粪便样品进行毒素检测。结果 206份粪便滤液经细胞毒检测有26份样品使非洲绿猴肾细胞(vero细胞)圆缩化,确认含有艰难梭菌毒素;经酶联免疫吸附试验有28份为阳性,其中23份与细胞毒检测结果一致,与细胞毒试验的符合率为88.5%。结论兰州地区住院腹泻患者中艰难梭菌感染率约为12.62%。

Objective To investigate the characteristics of Clostridium difficile in Lanzhou area and reveal current situation of the infection. Methods Toxin detection was performed on 206 fecal samples by using cytotoxicity detection and enzyme-linked immunity method. Results 26 out of the 206 samples made the African green monkey’s kidney（ vero） cells to shorten and turn round,indicating the existence of Clostridium difficile toxin. 28 samples were positive by enzyme-linked immunosorbent assay,among which 23 samples had the same results with those by cell toxin detection,and the coincidence rate was 88. 5%. Conclusion The infection rate of Clostridium difficile in hospitalized patients with diarrhea in Lanzhou area is about 12. 62%.