游泳运动对胰岛素抵抗大鼠血清炎性细胞因子的影响研究

Effects of swimming on the serum inflammatory cytokines of insulin resistance rats

研究游泳运动对RBP4诱导的胰岛素抵抗大鼠血清炎性细胞因子及免疫的影响。8周龄雄性SD大鼠腹腔注射3μg·g-1体重的重组RBP4,每天2次,每次间隔12 h,总共持续注射6周。设RBP4运动组(RE)、RBP4安静组(RR)和正常安静对照组(C),RE组给予6周无负重游泳训练,60 min·d-1。运用双抗体夹心酶标免疫分析法测定RBP4;免疫印迹法检测胰岛素抵抗指数(HOMA-IR);TUNEL法标记各组大鼠胸腺的凋亡细胞;ELISA检测血清IL-6的含量;双抗体夹心ABC-ELISA法检测血清GM-CSF含量。RE组血清RBP4和HOMA-IR显著低于RR组(P<0.01);RE组胸腺细胞凋亡相对RR组减少;RE组与RR组大鼠血清IL-6含量降低(P<0.01),但RR组中血清IL-6和血清GM-CSF的含量显著地高于C组(P<0.01);IL-6、GM-CSF与HOMA-IR皆呈中度正相关。

Effects of swimming on immunity of RBP4-induced insulin resistance to serum inflamma- tory cytokines in rats were studied. Eight-week-old SD male rats were given intraperitoneal injection of RBP4 at a dose of 3 μg·g^-1, twice a day for six weeks. These rats were divided into three groups： RBP4 ＋ swimming group （RE） , RBP4 ＋ rest group （ RR）, and normal control group （ C ）. RE group rats swam without load one hour per day for six weeks. The quantitative sandwich enzyme immunoassay technique was employed to detect RBP4 and the western blotting to detect HOMA-IR. The TUNEL meth- od was used to mark the apoptotic cells in rat thymus for all the rats. The ELISA method was used to de- tect serum IL-6 contents. The ABC-ELISA method was used to test GM-CSF in blood serum. Serum RBP4 and HOMA-IR in RE group rats were significantly lower than ones in RR group rats （P 〈0.01 ）. The apoptotic thymus cells in RE group rats were less than ones in RR group rats. Serum IL-6 contents in RE and RR groups reduced（P 〈0.01 ） while the contents of serum IL-6 and GM-CSF in RR group were obviously higher than the ones in C group （P 〈 0.01 ）. IL-6, GM-CSF and HOMA-IR were moderately correlated.