番茄bZIP转录因子家族的生物信息学分析

Comprehensive bioinformatic analysis of bZIP transcription factors in Solanum lycopersicum

bZIP转录因子广泛参与了植物生长发育以及抗逆应答的调控,属于重要的转录因子家族.为了系统地阐明番茄bZIP转录因子家族成员特征和基因表达模式,利用生物信息学软件和方法,对番茄基因组中的bZIP转录因子进行了系统的分析研究.结果表明:番茄基因组共编码70个bZIP转录因子基因,划分为14个亚族,即A、B、C、D、E、F、G、H、I、J、K、L、S和X.番茄、拟南芥和水稻bZIP基因的系统进化树揭示了进化过程中Group 3和Group 4的成员数目大大增加,进化过程较激烈.氨基酸保守域DOG1和MFMR分别特定存在于12个和6个bZIP成员蛋白序列中.在番茄各器官组织中,干旱、盐、细菌和病毒侵染胁迫条件下,43个番茄bZIP基因不同程度地被诱导表达.以基因表达值上调或下调1.5倍为阈值,部分bZIP基因的表达水平被显著地诱导上调或下调表达.这些基因很可能参与了调控番茄的果实等组织的生长发育,以及逆境胁迫条件下的防御应答反应.通过荧光实时定量PCR,其中7个代表性番茄bZIP基因,被进一步验证了在叶片和果实中的表达水平.本研究鉴定得到的番茄bZIP转录因子基因,可用于今后番茄育种及改良的基因工程研究.

The bZIP transcription factors are important participants in regulating plant development and defense response against various biotic and abiotic stresses. The characterization and expression pattern of tomato bZIP transcription factors are not clear so far therefore needing systematic analysis. Using the bioinformatics softwares and methods, we performed comprehensive bioinformatic analysis for the bZIP transcription factors in Solanum lycopersicum. All together 70 tomato bZIP transcription factors were identified and classified into 14 groups, including A, B, C, D, E, F, G, H, I, J, K, L, S and X. The phylogenetic tree of bZIP genes among tomato, arabidopsis and rice demonstrated that the members in Group 3 and Group 4 largely expanded, implying sharp evolution process. In 12 and 6 tomato bZIP transcription factors, DOG1 and MFMR conserved motif existed, respectively. Furthermore, 43 tomato bZIP genes showed distinct expression patterns in various tomato organs and tissues, as well as under stresses of drought, salt, bacteria and virus invasion. Under the criteria of change ration（1.5）, the expressions of several tomato bZIP genes were significantly up/down-regulated, implying these members might participate in regulating tomato development, and the defense response against various biotic and abiotic stresses. The expression of 7 representative tomato bZIP genes in different tissues was validated by real time quantitative RT-PCR. Our study could provide bZIP genes for further genetic engineering study of tomato breeding and improvement.