鞘内注射单羧酸转运蛋白抑制剂α-氰基-4-羟基肉桂酸缓解大鼠神经病理性疼痛

Intrathecal injection of monocarboxylate transporter inhibitor α-cyano-4-hydroxy-cinnamate attenuates neuropathic pain in rats

目的 观察鞘内注射4-CIN对坐骨神经慢性缩窄性损伤（CCI）大鼠神经病理性疼痛的影响.方法 18只雄性SD大鼠按随机数字表法分为3组（n=6）：假手术组（S组）、CCI组（C0组）及4-CIN组（C1组）.C0组与C1组建立CCI动物模型,S组仅分离而不结扎坐骨神经.术后第2天,C1组鞘内注射溶解于10％二甲基亚砜（DMSO） 10μl中的4-CIN（α-cyano-4-hydroxy-cinnamate）100 μmol,C0组仅鞘内注射DMSO10μl.各组在术前1d、术后第1,3,7,10,14天（T0-5）测定大鼠机械缩足反应阈值（PWMT）和热缩足反应潜伏期（PWTL）.结果 各组术前PWMT和PWTL差异无统计学意义（P＞0.05）.与S组相比,C0组在T1-5时PWMT[（11.71±2.81）g,（9.76±1.00）g,（8.22±1.33）g,（6.50± 1.48）g,（4.67± 1.03）g]、PWTL[（11.36±2.18）s,（11.60±2.54）s,（8.54±1.42）s,（7.59±1.00）s,（6.88±0.42）s]均出现明显降低（P＜0.05）,而C1组在给药后T2-5时与S组无明显差异（P＞0.05）.结论 鞘内注射4-CIN可缓解CCI所致的慢性神经病理性疼痛.

Objective To investigate the effects of intrathecal injection of α-cyano-4-hydroxy-cinnamate （4-CIN） in rats with neuropathic pain induced by chronic constriction injury of sciatic nerve （CCI）.Methods Eighteen male SD rats were divided randomly into 3 groups（n=6）：sham group （group S）,CCI model group （group C0） and 4-CIN group （group C1）.Group C0 and C1 were operated with the model of neuropathic pain induced by chronic constriction injury of sciatic nerve ; and group S were treated as sham operated rats.Two days after operation,group C1 received intrathecal injection of 100 μmol 4-CIN dissolved in 10% DMSO 10 μl,while group C0 received intrathecal injection of 10% DMSO 10 μl only.The paw withdrawal thermal latency（PWTL） and paw withdrawal mechanical threshold（PWMT） were tested 1 d before operation and 1 d,3 d,7 d,10 d,14 d after operation（T0-5）.Results The basic values of PWMT and PWTL before operation showed no statistically significant differences among the three groups.On T1-5,the PWMT（（11.71 ±2.81） g,（9.76± 1.00） g,（8.22± 1.33） g,（6.50± 1.48） g,（4.67± 1.03） g） and PWTL（（11.36±2.18） s,（11.60±2.54） s,（8.54± 1.42） s,（7.59± 1.00） s,（6.88± 0.42） s） in group C0 were significantly lower than those in group S （P〈0.05）.However,there were no significant differences between group S and C1 on T2-5（P〉0.05）.Conclusion Intrathecal administration of 4-CIN can attenuate neuropathic pain in rats induced by CCI.