慢性氟中毒大鼠骨组织中AC-cAMP-PKA信号转导通路的表达

Expression of AC-cAMP-PKA Signal Transduction Pathway in Bone Tissue of Rats with Chronic Fluorosis

目的观察慢性氟中毒大鼠骨组织中AC、cAMP、PKA的表达。方法将48只断乳2周的Wistar大鼠按体重随机分为4组,分别为对照组(自来水)和低剂量组(含50 mg/L NaF自来水)、中剂量组(含150mg/L NaF自来水)、高剂量组(含250 mg/L NaF自来水),每组12只,雌雄各半。采用自由饮用的方式进行染毒,连续染毒6个月。检测大鼠尿氟、骨氟浓度及氟斑牙发生率以及骨组织中AC、cAMP、PKA的水平。结果与对照组比较,各剂量染毒组大鼠尿氟、牙氟及骨氟浓度均较高(P<0.05),斑牙检出率随染毒时间延长而增高,随染毒剂量的增加氟斑牙程度越严重;而各组骨组织中AC、cAMP、PKA含量差异无统计学意义(P>0.05)。结论在本次实验中未观察到氟化钠引起大鼠骨组织AC、cAMP含量的改变。

Objectives To observe the expression of AC,cAMP and PKA in bone tissue of rats with chronic fluorosis. Methods Forty-eight Wistar rats at 2 weeks after weaning were randomly divided into 4 groups according to body weight： a control group（treated with tap water） and 3 NaF exposure groups（treated with NaF in drinking water at 50 mg /L,150 mg /L and 250 mg /L）. There were 6 female rats and 6 male rats in each group.The rats were executed after 6 months of treatment. The concentration of fluoride in bone tissue and urine,the incidence rate of dental fluorosis,and the levels of AC,cAMP and PKA in bone tissue were assayed. Results The concentrations of fluoride in bone tissue,urine and tooth for each fluoride exposure group were significantly higher than the control group（P 〈 0. 05）. Compared with the control group,the incidence of dental fluorosis in all exposure groups was obviously higher in a dose-dependent and exposure time-dependent manner. There was no significant difference on AC,cAMP and PKA in the bone tissue of rats in each group（P 〉 0. 05）. Conclusions There was no change on the levels of AC,cAMP in the bone tissue of rats caused by sodium fluoride in the present study.