miR-200c过表达对OVCAR-3卵巢癌细胞生物学行为的影响

The Effect of miR-200c Over Expression on OVCAR-3 Epithelial Ovarian Cancer Biological Functions

目的:探讨miR-200c过表达对上皮性卵巢癌细胞OVCAR-3增殖、迁移和侵袭能力的影响。方法:构建逆转录病毒表达质粒pMSCVpuro-miR-200c,转染OVCAR-3卵巢癌细胞株,分别用细胞计数试剂盒(Cell Counting Kit-8,CCK8)和Transwell小室法检测过表达miR-200c的OVCAR-3细胞的增殖、迁移以及侵袭力改变,同时采用基因芯片检测转染前后OVCAR-3细胞的差异基因表达情况。结果:与未转染细胞相比,pMSCVpuro-miR-200c-OVCAR-3细胞的增殖、迁移及侵袭力下降(均P<0.01)。通过基因芯片的检测,miR-200c过表达可引起OVCAR-3细胞中上调表达的基因有190个,下调表达的基因有166个。结论:miR-200c作为抑癌基因参与抑制上皮卵巢癌细胞的增殖、迁移和侵袭力。

Objective： To investigate the effect of the miR-200c over-expression on the ability of proliferation, migration and invasion in OVCAR-3 ovarian cancer cells. Methods：Constructed retrovirus expression vector pMSCVpuro-miR-200c and transfected OVCAR-3 cells to overexpress miR-200c. By using CCK8 and Transwell methods to detect the ability of OVCAR-3 cells in proliferation and metastasis. Different expression genes involved in OVCAR-3 cells were detected by using gene chip before and after transfected with pMSCVpuro-miR-200c. Results：Compared with untransfected cells, the ability of proliferation, migration and invasion in pMSCVpuro-miR-200c transfected OVCAR-3 cell are significantly decreased （P〈0.01）. 190 up-regulated genes and 166 down-regulated genes were detected in OVCAR-3 cells after over-expressed miR-200c. Conclusions：As a tumor suppression gene,overexpression of miR-200c could inhibit the proliferation,migration and invasion of ovarian cancer cells.