磁性聚合物微球固定化青霉素G酰化酶制备光学纯(S)-2-氯苯甘氨酸

Enzymatic preparation of optically pure(S)-2-chlorophenylglycine by magnetic immobilized penicillin G acylase

通过反相悬浮聚合法制备了超顺磁性环氧聚合物微球用于固定化青霉素G酰化酶,利用磁性固定化酶催化N-苯乙酰-(R,S)-2-氯苯甘氨酸进行不对称水解反应,制备出(S)-2-氯苯甘氨酸单一对映体。磁性固定化酶催化水解反应的适宜条件为:底物浓度100 mg·m L-1,反应温度和时间30℃和12 h,反应溶液p H 8.0。在此条件下,N-苯乙酰-(R,S)-2-氯苯甘氨酸的转化率为48.8%,产物(S)-2-氯苯甘氨酸的对映体过量值eep达99.4%。磁场下回收磁性固定化青霉素G酰化酶,重复使用6次,底物的转化率和产物的对映体过量值分别为47.8%和91.4%。

The prepared polymer microspheres with epoxy groups were super paramagnetic by inverse suspension polymerization forimmobilizing penicillin G acylase. （S）-2-chlorophenylglycine was synthesized via asymmetric hydrolysis of N-phenylacetic-（R,S）-2-chlorophenylglycine using the magnetic immobilized penicillin G acylase. Under the optimum reaction conditions of the substrateconcentration 100 mg·mL-1 ,temperature 30℃,pH 8. 0,reaction time 12 h,the conversion of N-phenylacetic-（R,S）-2-chlorophenylglycinewas 48. 8%,the enantiomeric excess（eep）of（S）-2-chlorophenylglycine was 99. 4%. The conversion of substrate and enantiomericexcess（eep）of product was 47. 8%,and 91. 4%respectively by recovering the immobilized penicillin G acylase in magneticfield in six recycling tests.