垂丝海棠叶多糖的超声提取及其抗氧化性质研究

Study on ultrasonic extraction process of polysaccharides and antioxidant activities from the leaves of malus halliana koehne

在单因素实验的基础上,采用Box-Behnken中心组合实验和响应面分析法,研究提取时间、超声波功率、液料比对垂丝海棠叶多糖含量的影响,建立影响因素与响应值之间的数学模型,确立最佳提取工艺为:提取时间20 min,液料比45∶1,超声波功率135W。抗氧化实验结果表明,在达到最大浓度0.74mg·m L-1时,垂丝海棠叶多糖(超声)、垂丝海棠叶多糖(煮沸)和Vc的对DPPH的清除率依次为79.9%,69.7%,64.8%,垂丝海棠叶多糖(超声)和垂丝海棠叶多糖(煮沸)对·OH自由基的清除率分别为60.1%、51.2%,垂丝海棠叶多糖(超声)还原Fe3+能力较强。

According to single-factor experiments,Box-Behnken design and its response surface analysis,main factors that affectaedthe polysaccharides yield from the leaves in Malus halliana koehne such as extraction time,ultrasonic power,liquid-material ratiowere studied to find the optimal process. The best extraction process was as follows：liquid-material ratio was 45 ∶ 1,extracting 20min by 135W power of ultrasound. According to the results of antioxidant activities,the DPPH scavenging activities of polysaccharidesextracted by ultrasound,polysaccharides extracted by boiling method and vitamin C was respectively 79. 9%,69. 7%,64. 8%when concentration of the polysaccharides reached the 0. 74mg·mL-1 . Scavenging activities of free radical·OH of the polysaccharidesextracted by ultrasound and boiling method was respectively 60. 1% and 51. 2%. Meanwhile,the polysaccharides from ultrasonicextraction revealed their better Fe3＋ reduction activities.