L-色氨酸在金纳米粒子修饰电极上的电化学行为及其分析研究

Electrochemical behavior and analysis of L-tryptophan on the Au nanoparticle modified glassy carbon electrode

制备了金纳米粒子修饰玻碳电极（Au/ GCE）,用循环伏安法研究L-色氨酸（L-Trp）在修饰电极上的电化学行为,以及支持电解质、溶液pH、扫描速率等对L-Trp 伏安响应的影响.实验表明：在pH = 3. 5 的HAc-NaAc 支持电解质中,L-Trp 在Au/ GCE 上有一灵敏的氧化峰（Epa =0. 93）.氧化峰电流与L-Trp 浓度在5. 0×10^-7-1. 0×10^-4 mol·L^-1 范围内呈良好的线性关系,相关系数为0. 9990,检出限1. 6×10^-7 mol·L^-1 .测得L-Trp样品平均回收率为98%.

Electrochemical behavior of L-Trp on gold Nanoparticles modified glassy carbon electrode（GCE）was investigated by cyclicvoltammetry（CV）. Effect of different buffer solution,pH value and scanning rate on electrode performance was investigated. Resultsshow that L-Trp has an irreversible oxidation peak at 0. 93V（vs. SCE）in HAc-NaAc buffer solution. The peak current at Au/GCE is much higher than that at bare GCE. Furthermore,the proposed sensor shows a rapid and highly sensitive linear response toL-Trp in range of 5. 0×10-7 -1. 0×10-4 mol·L-1 with a detection limit of 1. 6×10-7 mol·L-1（relative coefficient=0. 9990）and a recoveryof 98%.