淋球菌耐药性分析及相关耐药基因检测

Drug resistance analysis of Neisseria gonorrhoeae and its related drug resistant gene detection

目的了解佛山地区淋球菌对5种抗菌药物的耐药性,并检测相关耐药基因突变情况。方法收集57株淋球菌,药敏试验采用琼脂稀释法。PCR法扩增相关耐药基因,PCR产物测序结果在GenBank中用blastn进行核酸序列同源性搜索。结果淋球菌对青霉素、四环素、环丙沙星、头孢曲松、大观霉素的敏感率分别为0.0%、8.8%、7.0%、61.4%、100.0%,产β-内酰胺酶淋球菌和质粒介导的四环素高度耐药淋球菌阳性率分别为35.1%、56.1%。耐药基因突变率均在80%以上。结论头孢曲松、大观霉素可以作为佛山地区治疗淋球菌的首选药物。淋球菌耐药机制较复杂,应加强对相关耐药基因分子流行病学监测。

Objective To understand the drug resistance of Neisseria gonorrhoeae in Foshan area and to detect the related drug resistant gene mutation situation.Methods 57 strains of Neisseria gonorrhoeae were collected.The drug susceptibility test was per-formed by adopting the agar dilution method.The related drug resistance genes were amplified by PCR and the PCR product se-quencing results were performed the homological searching in GenBank by the BLAST algorithm.Results The sensitive rates of Neisseria gonorrhoeae to penicillin,tetracycline,ciprooxacin,ceftriaxone and spectinomycin were 0.0%,8.8%,7.0%,61.4% and 100.0%,respectively.The rates of beta lactamase-producing Neisseria gonorrhoeae and tetracycline resistant Neisseria gonorrhoeae were 35.1% and 56.1%,respectively.The mutation rate of drug resistance genes were over 80%.Conclusion Ceftriaxone and spectinomycin can be used as the first choice drug for the treatment of Neisseria gonorrhoeae in Foshan region.The drug resistance mechanism of Neisseria gonorrhoeae is complex.The epidemiological monitoring of the Neisseria gonorrhoeae related drug resist-ance genes should be strengthened.