罗氏Cobas e601与雅培Architect i2000检测乙肝表面抗原的比较

Comparison of HBsAg Results between Roche Cobas e601 and Abbott Architect i2000 analysis systems

目的比较罗氏电化学发光检测仪Cobas e601与雅培化学发光微粒子免疫分析仪Architect i2000检测乙肝表面抗原的结果。方法选取在雅培i2000检测仪上检测乙肝表面抗原结果为有反应性,且定量结果小于250IU/mL的标本,同时在罗氏e601进行检测,分析比较检测结果间的差别,并进行直线相关和回归分析。结果共检测46份临床标本,剔除1份两台仪器上检测结果反应性不符的标本外,其余结果具有很好的相关性。其中雅培检测值为0.05~1.00IU/mL的15份,两者的直线回归方程为Y=17.49 X＋0.843,相关系数r=0.979;1.1~10.00IU/mL的15份,两者的直线回归方程为Y=15.72 X＋21.06,相关系数r=0.952;11~250IU/mL的15份,两者的直线回归方程为Y=29.17 X-129,相关系数r=1.000。结论两种方法的检测结果具有很好的相关性,并可以通过公式进行相互换算。

Objective To compare the difference of the HBsAg detected results between the Roche cobas e601 electrochemilumi-nescence immunoassay instrument and the Abbott Architect i2000chemiluminescent microparticle immunoassay instrument.Methods The HBsAg positive specimens with the quantitation results of lower than 250IU/mL detected by the Abbott Architect i2000 were selected and simultaneously detected by the Roche cobas e601.The differences of detected results were compared and per-formed the linear correlation and analysis regression.Results 46 clinical specimens were detected.The detected results had best correlation between the two instruments by getting rid of 1 specimen with unconformable reactivity of detected results.15 speci-mens had the HBsAg detected result of 0.05-1.00 IU/mL by the Abbott Architect i2000,the linear regression equation was Y =17.49X＋0.843（r=0.979）;15 specimens had the HBsAg detected result of 1.1 -10.00 IU/mL IU/mL by the Abbott Architect i2000,the linear regression equation was Y =15.72X ＋21.06（r=0.952）;15 specimens had the HBsAg detected result of 11 -250 IU/mL by the Abbott Architect i2000,the linear regression equation was Y =29.17X -129（r=1.000）.Conclusion The detected results have better correlation between the two instruments and can be mutually converted by the formulas.