一株多环芳烃芘降解菌的鉴定及其降解特性研究

Isolation and characterization of a pyrene-degrading marine bacterium from mangrove(Rhizophora stylosa)

从红树植物红海榄叶片中分离一株对芘具有较好降解作用的海洋细菌,命名为B11,并对其菌体特征、生长条件及降解效能等进行了系统研究。结果发现该菌株为革兰氏阴性菌,结合其生理生化特征和16S rDNA序列比对,表明此菌株属于交替单胞菌属(Alteromonas sp.)。菌株在芘的起始浓度为100 mg/L,pH为中性或偏碱性条件下,盐度35时对芘的降解作用最强。外加碳源对B11对芘的降解效果都具有一定的抑制作用,其中水杨酸的抑制作用更为显著。综合菌株B11的生长特性和降解效能,初步认为B11菌株较适合用于降解多环芳烃芘,可用于红树植物多环芳烃生态污染的原位修复。

A predominant indigenous bacterium utilizing pyrene as a sole carbon and energy source was isolated from mangrove （Rhizophora stylosa） in Zhanjiang, China. The morphology, physiological and biochemcial characteristics, capacity of degradation were studied. The results showed that the strain was Gram-negative bacteria . Based on its characteristics mentioned above as well as the 16 S rDNA sequence analysis, the bacterium was identified as Alteromonas sp., and named B11. The best pyrene degradation condition of B11 was that the initial concentration of pyrene was 100 mg/L, pH was 7 or a little alkalinity and salinity was almost 35. Extra carbon source especially salicylic acid reduced degradation rate of pyrene by B11 strain. The results showed that strain B11 was suitable to degrade Pyrene and indigenous rehabilitation in mangrove polluted zone.