蕲艾提取液药物血清对大鼠肝星状细胞增殖的影响

Effect of Artemisia Argyi Extract Solution Drug Serum on Proliferation of Rat's Hepatic Stellate Cells

目的研究蕲艾提取液药物血清对大鼠肝星状细胞(HSC)增殖及转化生长因子β1(TGFβ1)表达的影响。方法传代培养的HSC-T6与蕲艾提取液药物血清共同培养24h后,采用MTT法检测其对HSC增殖的影响,Elisa法检测TGFβ1蛋白表达情况。结果不同浓度蕲艾提取液药物血清(5%、10%、20%)与空白对照组及生理盐水血清组比较,能显著抑制HSC-T6增殖(P<0.01)及TGFβ1蛋白的表达(P<0.01)。结论蕲艾提取液药物血清可显著抑制HSC-T6增殖。

Objective To investigate the effect of Artemisia argyi extract solution drug serum on rat hepatic stellate cells （HSC） prolif- eration and the expression of TGFβ1. Methods HSC - T6 was incubated with different concentrations of Artemisia argyi extract solution drug serum （5% , 10% , 20% ） for 24 hours,cell proliferation was detected by MTT assay,and the expression of TGFβ1 was detected by Elisa. Results Different concentrations of Artemisia argyi extract solution drug serum （5%, 10%, 20% ） could significantly inhibit the prolifera- tion of HSC -T6 （P 〈 0.01 ）and the expression of TGFβ1 （P 〈 0.01 ）compared with blank control group and the saline group. Conclusion Artemisia argyi extract solution drug serum can significantly inhibit the proliferation of HSC - T6.