鳖甲水提液对小鼠急性酒精性肝损伤的保护作用

Protective Effect of Trionycis Carapax Aqueous Extract on Mice's Acute Alcohol-induced Liver Injury

目的采用小鼠急性酒精性肝损伤模型,探讨鳖甲水提液对小鼠急性酒精性肝损伤的保护作用及其机制。方法昆明(KM)小鼠随机分为正常组,模型组,联苯双酯组(200mg/kg),鳖甲水提液高、中、低剂量组(相当于生药材36g/kg、18g/kg、9g/kg)。各给药组给药30d,第30d模型组及各给药组采用50%乙醇一次灌胃制备小鼠急性酒精性肝损伤模型。测定小鼠血清ALT、AST值,肝组织匀浆测TG、MDA、SOD、ADH、GSH,并取肝脏做HE染色及苏丹III染色观察组织形态变化。结果与模型组相比,鳖甲水提液高剂量组ALT值显著降低,SOD活力、GSH含量显著升高;各剂量组肝组织中MDA含量、TG浓度显著降低;鳖甲水提液高,中剂量组ADH活力显著增加。结论鳖甲水提液对小鼠急性酒精性肝损伤具有保护作用,其作用机制可能与清除体内氧自由基、抗脂质过氧化及促进酒精在体内的代谢有关。

Objective Using the model of acute alcohol - induced liver injury to explore the protective effect of Trionycis Carapax aque- ous extract on it and its mechanism. Method KM mice were randomly divided into 6 groups including normal group, bifendate group, Tri- onycis Carapax aqueous extract high dose group,Trionycis Carapax aqueous extract medium dose group ,Trionycis Carapax aqueous extract low dose group, each group was respectively treated for 30days. On 30th day,all groups（except normal group） used 50% alcohol to induce acute alcoholic liver injury in mice. ALT and AST were detected in the mice serum;SOD, TG, MDA, ADH were detected in the liver tissue. And HE and Sudan Ⅲ staining were used to observe the morphological changes of liver tissue in mice. Result Compared with model group, the ALT values of high dose group reduced significantly, SOD activity and GSH concentration increased significantly. All groups of MDA concen- tration , TG concentration in liver tissue decreased significantly; the ADH activity of high dose group and medium dose group of Trionycis Carapax aqueous extract increased significantly. Conclusion The Trionycis Carapax aqueous extract have protective effect on acute alcoholic liver injury in mice, Its mechanism may be related to remove oxygen free radicals, promote the metabolism of alcohol, fight against lipid peroxi- dation in the body.