水杨酸和盐浸种对香稻和非香稻幼苗生理特性的影响

Effects of Pre-soaking with Salicylic Acid and Salt on Some Physiological Characteristics of the Aromatic and Non-aromatic Rice Seedlings

为探明水杨酸和盐对香稻和非香稻幼苗生理特性的影响,以香稻品种农香18和非香稻品种华航丝苗为材料,设置水杨酸(SA)0,1 mmol/L和盐(NaCl)0,50 mmol/L浸种处理,测定了幼苗叶片和茎鞘超氧化物超化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、可溶性蛋白含量、脯氨酸含量及香稻香气2-AP含量。SA、盐以及两者的混合溶液浸后,香稻2-AP含量均显著增加。1 mmol/L水杨酸与50 mmol/L盐混合溶液处理显著提高香稻幼苗脯氨酸含量而对非香稻品种无显著影响;50 mmol/L盐溶液处理可显著提高香稻和非香稻幼苗茎鞘POD活性及香稻幼苗茎鞘SOD活性;1 mmol/L水杨酸处理显著提高香稻和非香稻幼苗叶片脯氨酸含量及香稻幼苗POD活性。SOD、脯氨酸和POD是水稻幼苗响应水杨酸和盐浸种处理的主要生理特性指标。盐胁迫下香稻2-AP含量增加,SA可缓解盐胁迫,同时可提高香稻2-AP含量。

The purpose of this study was to investigate the effects of salicylic acid（ SA） and salt on some physiological characteristics of aromatic and non-aromatic rice seedlings. Aromatic rice Nongxiang 18 and non-aromatic rice Huahangsimiao were used as materials,seed pre-soaking treatments were SA treatment（ Seeds soaked in 0 and1 mmol /L of SA） and salt treatment（ Seeds soaked in 0 and 50 mmol /L of NaCl）. The SOD activity,POD activity,soluble protein content,proline content,MDA content in leaves and culm and sheath of rice seedling and 2-AP content in leaves and culm and sheath of aromatic rice were studied. The results showed that SA,NaCl and SA ＋ NaCl treatments significantly increased 2-AP content in aromatic rice seedling. The 1 mmol /L SA ＋ 50 mmol /L NaCl treatment significantly increased proline content in aromatic rice seedling,no significant difference was observed in non-aromatic rice; 50 mmol /L NaCl treatment significantly increased POD in culm and sheath of aromatic rice and non-aromatic rice seedling,and significantly increased SOD increased in aromatic rice seedling; 1 mmol /L SA treatment significantly increased proline content in leaves of both aromatic rice and non-aromatic rice seedling,and sig-nificantly increased POD activity in aromatic rice seedlings. These results indicated that the SOD,POD activity and proline content in rice seedlings were the main index respond to pre-soaking by SA and salt. SA relived salt stress and significantly increased 2-AP.