林蛙皮中胶原蛋白的含量测定研究

Determination of collagen content in the skin of rana chensinensis

目的扩大中国林蛙应用范围,增加其自身经济价值。建立林蛙皮中胶原蛋白的含量测定方法,并考察其中的胶原蛋白含量。方法应用Worssner比色法测定中国林蛙皮水解液中的L-羟脯氨酸的含量,计算出中国林蛙皮中胶原蛋白的含量。结果中国林蛙皮中L-羟脯氨酸的含量为37.7 mg/g,胶原蛋白在林蛙皮中百分比为37.86%。结论 Worssner比色法稳定、有效,可用于测定中国林蛙皮中胶原蛋白的含量测定。林蛙皮中胶原蛋白含量极高,可以作为新的提取来源。

Objective In order to expand the application of Rana Temporaria Chensinensis and increase its value,a method was established for determination and evaluation of the collagen content in the skin of Rana Chensinensis.Methods The L-hydroxyproline content in the hydrolysate of Rana Temporaria Chensinensis was detected by the method of Worssner colorimetric and the collagen content in the skin of Rana Chensinensis was speculated.Results The L-hydroxyproline content in the skin of Rana Chensinensis was 37.7 mg /g,and the percentage of collagen content in the skin of Rana Chensinensis was 37.86%.Conclusion The method of Worssner colorimetric was stable and efficient and could be used to determinate the collagen content in the skin of Rana Chensinensis.The skin of Rana Chensinensis was rich in collagen,and could be a new resource to be extracted.