Curcumin联合放射对人结肠癌细胞株HT29的增殖抑制作用研究

Influence of curcumin combined with radiotherapy on the proliferation and apoptosis of HT29 cells

目的探讨姜黄素(Curcumin,Cur)在放射线诱导的HT29细胞增殖及凋亡中的作用。方法常规培养HT29细胞,经6 Gy放射线照射和(或)Cur处理24 h后,通过MTT比色法分析空白对照组、6 Gy单独照射组及联合处理组(5μmol/L姜黄素+6 Gy组、10μmol/L姜黄素+6 Gy组、20μmol/L姜黄素+6 Gy组)HT29细胞增殖率,采用流式细胞术Annexin V-FITC/PI双染法分析细胞凋亡率,通过Elisa实验评价细胞中半胱氨酸蛋白酶Caspase-3、Caspase-6和Caspase-9活性。结果 Cur和6 Gy联合处理组的细胞增殖率明显高于空白对照组和6 Gy单独照射组(P<0.05),细胞凋亡率亦显著高于空白对照组和6 Gy单独照射组(P<0.01);同时,Cur和6 Gy联合处理组细胞中Caspase-3、Caspase-6和Caspase-9活性亦明显高于对照组和6 Gy单独照射组(P<0.05)。结论 Cur可通过促进细胞凋亡相关因子活性,进而诱导HT29细胞凋亡,抑制其增殖,发挥HT29细胞对放射线的增敏作用,此可为结肠癌放射治疗的临床研究提供新的思路。

Objective To explore the effects of curcumin（Cur） on the proliferation and apoptosis of HT29 cells induced by radiotherapy. Methods HT29 cells were routinely cultured and treated with 6 Gy and（or） Cur for24 h： control group, 6 Gy singly used group,6 Gy combined with Cur group（5 μmol/L Cur＋6Gy, 10 μmol/L Cur＋6Gy,20 μmol/L Cur＋6Gy）. Then MTT assay, flow cytometer, annexin V-FITC/PI double staining and elisa assay were performed to evaluate the proliferating ratio, apoptotic ratio and assess the Caspase-3, Caspase-6 and Caspase-9 activities in HT29 cells. Results The cell proliferating ratio of HT29 cells in 6 Gy combined with Cur group was significantly decreased compared to the corresponding cells in either control or 6 Gy singly used groups（P〈0.05）, and the apoptotic levels of HT29 cells were significantly increased compared to the corresponding cells in either control or6 Gy singly used groups（P〈0.01）. Simultaneously, the Caspase-3, Caspase-6 and Caspase-9 activities in the cells in6 Gy combined with Cur group were increased compared to the corresponding cells in control and 6 Gy singly used groups（P〈0.05）. Conclusion Cur can increase the sensitivity of HT29 cells in response to 6 Gy through induction of apoptosis-associated factors and increasing apoptosis. Thus, our study may provide a new concern for the clinical treatment of colon carcinoma.